Method of reconstituting nucleic acid molecules

Details Method of reconstituting nucleic acid molecules Method of reconstituting nucleic acid molecules

2005-03-29

2005-03-29

McKelvey, Terry (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091100

Reexamination Certificate

active

06872552

ABSTRACT:
The present invention provides a system for reconstituting nucleic acid molecules that have been degraded but still contain useful genetic information. The present invention uses, as a template for reconstituting degraded nucleic acids in a biological sample, nucleic acids from a genetically related or identical organism having a sequence homologous to the degraded nucleic acids. After hybridization of the degraded nucleic acids to the template, regions of the degraded nucleic acids that are missing in the duplex containing template nucleic acid molecules hybridized to degraded nucleic acids are filled in with nucleotides using the intact nucleic acid molecule as a template. The newly formed strand of nucleic acid is used as the template for a subsequent step of hybridization to degraded nucleic acid molecules. Regions of degraded nucleic acids that are missing in the duplex are again filled in with nucleotides using the newly formed nucleic acid as the new template. The process is repeated until the newly formed nucleic acid molecules are substantially representative of the nucleic acids from the genome of the species from which the degraded sample is obtained.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5750335 (1998-05-01), Gifford
patent: 0 721 016 (1996-07-01), None
patent: WO 9309250 (1993-05-01), None
patent: WO 9521271 (1995-08-01), None
patent: WO 9731256 (1997-08-01), None
patent: WO 9806830 (1998-02-01), None
patent: WO 0056927 (2000-09-01), None
Skryabin et al., Nucleic Acids Research, vol. 18, No. 14, 4289 (19990).*
Hsu, et al., “Visualization of Novel Simian Virus 40 DNA Recombination Intermediates Induced by Ultraviolet Light Irradiation”,Nucleic Acids Research, 19(25): 7193-7199, 1991.
Komori, et al., “A Holliday Junction Resolvase from Pyrococcus Furiosus: Functional Similarity toEscherichia coliRuvC Provides Evidence for Conserved Mechanism of Homologous Recombination in Bacteria, Eukarya, and Archaea”,Proc. Natl. Acad. Sci., USA, 96: 8873-8878, 1999.
Majesky et al., “Rat Carotid Neointimal Smooth Muscle Cells Reexpress a Developmentally Regulated mRNA Phenotype During Repair of Arterial Injury”, XP-000995806.
Meselson, et al., “A General Model for Genetic Recombination”,Proc. Nat. Acad. Sci, USA, 72(1): 358-361, 1975.
Orr-Weaver, et al., “Yeast Transformation: A Model System for the Study of Recombination”,Proc. Natl. Acad. Sci. USA, 78(10): 6354-6358, 1981.
Sekiguchi, et al., “Resolution of Holliday Junctions by Eukaryotic DNA Topoisomerase I”,Proc. Natl. Acad. Sci. USA, 93: 785-789, 1996.
Szostak, et al., “The Double-Strand-Break Repair Model of Recombination”,Cell. 33: 25-35, 1983.
Vrhovski, et al., “Biochemistry of Tropoelastin”, Review, XP-000996163.
International Search Report issued for corresponding PCT application PCT/US01/06427.

Affiliated with

Also associated with

Rating

Method of reconstituting nucleic acid molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of reconstituting nucleic acid molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of reconstituting nucleic acid molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3444382