Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid
Patent
1984-09-11
1988-10-18
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving uric acid
435 11, 435 12, 435 28, 435 14, 435904, 436 66, 436 71, 436135, 436164, C12Q 126, C12Q 128, C12Q 154, C12Q 160
Patent
active
047787538
ABSTRACT:
Disclosed herein is a method of quantitatively measuring an oxidative substance with avoiding influences of a coloring-interfering substance in the quantitative measurement of the oxidative substance by using a triphenyl methane type leuco coloring matter as a coloring reagent. Disclosed herein is also such an oxidative quantitative measurement in which the coloring sensitivity can be further adjusted. In order to avoid the influences of the coloring-interfering substance, there is employed at least one kind of (i) uricase, (ii) an anionic surface active agent and (iii) a metal chelate compound. As the triphenyl methane type leuco coloring matter, use may be made of a compound of the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent a hydrogen atom or a lower alkyl group, and X.sub.1 and X.sub.2, which may be the same as or different from each other, represent a hydrogen atom, --SO.sub.3 M.sub.1, --COOM.sub.2, --O(CH.sub.2).sub.m SO.sub.3 M.sub.3, --O(CH.sub.2).sub.n COOM.sub.4 or --N(R.sub.5) (R.sub.6) in which M.sub.1, M.sub.2, M.sub.3 and M.sub.4 represent a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, R.sub.5 and R.sub.6 independently represent a hydrogen atom or a lower alkyl group, and m and n represent an integer of 2-4. Azide compound is further added or the compound represented by the general formula (I) is included with cyclodextrin or modified dextrine to adjust coloring sensitivity.
REFERENCES:
patent: 2850359 (1958-09-01), Worthington et al.
patent: 4317878 (1982-03-01), Nakanishi et al.
patent: 4330476 (1982-05-01), Hermann
patent: 4433060 (1984-02-01), Frenzel
patent: 4554249 (1985-11-01), Kosaka
patent: 4673635 (1987-06-01), Yamanishi et al.
Hanada Toshiro
Yamanishi Kazuhiko
Wako Pure Chemical Industries Ltd.
Warden Robert J.
Wieder Stephen C.
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