Method of quantitative enzyme determination

Details Method of quantitative enzyme determination Method of quantitative enzyme determination

1992-08-24

1997-03-18

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

422 56, 435 15, 435 18, 435 25, 435805, C12Q 100, C12Q 148, C12Q 134, C12Q 126

Patent

active

056121784

ABSTRACT:
Methods and kits are provided for the quantitative determination of the presence of an enzyme analyte in a sample by determination of the rate of analyte catalyzed reaction on a chromatographic medium. The methods may be used to determine both steady state and non-steady state enzyme reaction kinetics and provide a physical record reflecting the same. In a preferred embodiment, an analyte enzyme in a sample to be analyzed is immobilized at a reaction site on a chromatographic medium, the chromatographic medium is contacted with a solution containing a substrate, the solution is transported on the chromatographic medium to the reaction site where the analyte enzyme catalyzes the reaction of the substrate to produce a detectable reaction product at a rate related to the amount of enzyme present, and the solution and reaction product are transported from the reaction site to a detection region formed by a length of the chromatographic medium downstream from the reaction site. Transport continues until the solution reaches the end of the chromatographic medium or until the quantity of solution is exhausted.

REFERENCES:
patent: 3875014 (1975-04-01), Forgione
patent: 4042335 (1977-08-01), Clement
patent: 4235601 (1980-04-01), Deutsch et al.
patent: 4298688 (1981-11-01), Kallies
patent: 4629688 (1986-12-01), Bolguslaski et al.

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