Method of quantitative determination utilizing enzyme aggregatio

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 28, 435972, 436501, 436536, 436537, 436819, G01N 33536, G01N 33542

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active

051302349

ABSTRACT:
A quantitative determination of a substance is performed in a homogeneous system based on a change in enzyme activity; differences between the activity of a free enzyme and that of an enzyme bound by an aggregation or chemical bonding are observed. A peroxidase-labeled antibody and antigen system is one of of the typical example. Since the reaction is effected in a homogeneous system, the amount of antigen can be easily measured by the difference of enzyme activity.

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Annals of Clinical Biochemistry 16, 221-239 (1979) M. J. O'Sullivan et al. "Enzyme immunoassay: a review".

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