Method of quantitative determination of substances using coumari

Details Method of quantitative determination of substances using coumari Method of quantitative determination of substances using coumari

1994-08-16

1998-12-22

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 793, C12Q 128

Patent

active

058517855

ABSTRACT:
The present invention relates to a method for quantitatively determining a peroxidation-active substance, hydrogen peroxide or a coumarin derivative represented by formula (I) or (II): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 are the same or different and each represents hydrogen, substituted or unsubstituted lower alkyl, substituted or unsubstituted aralkyl, substituted or unsubstituted aryl or halogen; the method comprising reacting the hydrogen peroxide with the coumarin derivative represented by formula (I) or (II) or its salt in the presence of the peroxidation-active substance, and determining the amount of the light emission or the light intensity from the reaction solution.

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