Method of quantitative determination of peroxide, a peroxidation

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 25, 548255, 546 1, C12Q 128

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active

058010084

ABSTRACT:
Disclosed is a chemiluminescent method of quantitatively determining one of peroxide, a peroxidation-active substance or a pyrazolopyridopyridazine derivative represented by the formula (I): ##STR1## wherein R.sup.1 represents hydrogen, lower alkyl, optionally substituted aryl, optionally substituted heterocyclic group, substituted sulfonyl, substituted sulfinyl, substituted thio etc.; R.sup.2 represents hydrogen, lower alkyl, optionally substituted aryl, etc., or a salt thereof; characterized by subjecting an unknown amount of one substance selected from the peroxide, peroxidation-active substance and pyrazolopyridopyridazine derivative to react with a known amount of the remaining two substances.

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