Method of quantitative determination of bilirubin and a reagent

Details Method of quantitative determination of bilirubin and a reagent Method of quantitative determination of bilirubin and a reagent

1996-10-22

1999-01-12

Hollinden, Gary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

536 111, 435 4, C12Q 126

Patent

active

058586954

ABSTRACT:
Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.

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Clinical Chemistry, vol. 38, No. 2 (Feb. 1992) pp. 298-302.
Clinical Chemistry, vol. 34, No. 12, (Dec. 1988) p. 2581.

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