Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1997-12-01
1999-02-16
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 14, 435 4, C12Q 132, C12Q 154, C12Q 100
Patent
active
058719490
ABSTRACT:
Using a 1,5-anhydroglucitol dehydrogenase capable of acting on 1,5-anhydroglucitol and directly catalyzing a reducing chromophoric agent in the absence of an electron carrier, the 1,5-anhydroglucitol dehydrogenase is allowed to act on 1,5-anhydroglucitol in the presence of the reducing chromophoric agent preferably after the glucose in the specimen has been changed, or while being changed, into such a structure that it does not react with the 1,5-anhydroglucitol dehydrogenase in the specimen, by the aid of a glucose eliminator. Then, the amount of the resultant reduced colored substance is measured. As the 1,5-anhydroglucitol dehydrogenase, an enzyme produced by a microorganism having the ability to produce the 1,5-anhydroglucitol dehydrogenase is preferably used.
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Ebinuma Hiroyuki
Ushizawa Koji
Daiichi Pure Chemicals Co. Ltd.
Leary Louise N.
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