Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1988-10-26
1990-06-12
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435810, 436135, 436 84, 436 66, 436164, C12Q 128, G01N 3372, C12P 762
Patent
active
049332775
ABSTRACT:
A method for quantitative analysis of hydrogen peroxide at a high precision is disclosed. The method comprises reacting a divalent cobalt compound with hydrogen peroxide in the presence of peroxidase or a substance similar to peroxidase to produce a trivalent cobalt compound, reacting the trivalent cobalt compound thus produced with a trivalent cobalt indicator to produced a colored complex, and subjecting the colored complex to colorimetric quantitative analysis. The method enables various enzymatic activities producing hydrogen peroxide by the reaction with substrates as well as the amount or activity of substances involving the known enzymatic reactions linked with such enzymatic reactions, of enzymes and coenzymes, to be quantitatively determined very easily at a high sensitivity at a wavelength of above 600 nm without the interference of hemoglobin, bilirubin, or turbidity.
REFERENCES:
Yandell et al, Biochim. Biophys. Acta, 748: 263-270 "Steady-State Kinetics of Yeast Cytochrome c Peroxidase Catalyzed Oxidation of Inorganic Reductants by Hydrogen Peroxide", (1983).
Wada et al, Chemical Abstracts, 98: 227135v, p. 736 (1983).
Kina, "The Molecular Design of Highly Sensitive Photometric Reagent (1)", Dojin New, 39, 8-10, 1987.
Horiguchi, et al. "Water Soluble Pyridylazoaminophenols and Pyridylazo Amino Benzoic Acids as Highly Sensitive Photometric Reagents for zinc, uranium, cobalt and nickel."
Katami et al, "Spectrophotometric Determination of Cobalt in Pepperbush Leaves and Coal Fly Ashes Using 2-(2-Benzothiazolylazo)-5-Dimethylaminbenzoic Acid", Prefect. Res Inst. Environ. Pollut, 110(4), pp. 399-401.
Wada et al., "Spectrophotometric Determination of Cobalt in Steel with 2-(2-Thiazolylazo)-4-methyl-5-(Sulfomethylamino)Benzoic Acid (TAMSMB)", Mikrochim. Acta, 2(1-2), pp. 139-149, 1983.
Wada et al, "2-(2-Thiazolylazo)-4-Methyl-5-(Sulfomethylamino)Benzoic Acid as a Reagent for the Spectrophotometric Determination of Cobalt", Anal. Chim. Acta. (1982), 135(2), pp. 333-341.
Abe Toshikatsu
Izumi Atsushi
Maki Akemichi
Manabe Mitsuhisa
Masumi Fumio
Daiichi Pure Chemicals Co. Ltd.
Kepplinger Esther M.
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