Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Reexamination Certificate
2011-03-08
2011-03-08
Gakh, Yelena G (Department: 1797)
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
C435S023000
Reexamination Certificate
active
07901942
ABSTRACT:
A method is provided for quantifying a plasma membrane protein present by using a stable-isotope labeled peptide as a probe by mass spectrometry in a simple, quick and accurate manner. A plasma membrane protein is fragmented to prepare an oligopeptide fragment, identified by LC/MS/MS. A subject peptide for quantification is selected if the peptide is obtained by fragmenting with a protease, the peptide is specific to a target molecule, and if the peptide has a high total score value based on selective criteria for hydrophobic amino acids content, sequence conditions, number of amino acid residues, specific amino acid sequence conditions, etc. According to these criteria, a subject peptide fragment that can be ionized by ESI method is selected. By using the subject peptide for and a stable-isotope labeled peptide, the plasma membrane protein is quantified accurately by mass spectrometry.
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Kamiie Junichi
Otsuki Sumio
Terasaki Tetsuya
Adams Michelle M
Cadwalader Wickersham & Taft LLP
Gakh Yelena G
Tohoku University
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