Method of purifying toxin conjugates using hydrophobic interacti

Details Method of purifying toxin conjugates using hydrophobic interacti Method of purifying toxin conjugates using hydrophobic interacti

1986-10-10

1988-09-13

Kight, John

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, 530413, 530391, 530388, 530351, 530387, 530370, 424 85, 435 68, C07K 318

Patent

active

047711280

ABSTRACT:
A method of isolating and purifying toxin conjugates using hydrophobic interaction chromatography. Crude conjugate mixtures are sized to remove unconjugated toxin, and loaded onto a column filled with a suitable hydrophobic gel. Elution is effected with salt solutions of decreasing ionic strength, which salt solutions optionally include increasing amounts of an organic solvent. Toxin conjugate substantially free of unconjugated Ig and unconjugated toxin is provided.

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