Method of purifying human factor VIII

Details Method of purifying human factor VIII Method of purifying human factor VIII

1999-07-09

1999-12-07

Moezie, F. T.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, 530425, 530429, 530829, 436161, C07K 1700, C07K 1702

Patent

active

059985895

ABSTRACT:
The invention is a method of purifying Factor VIII comprising the steps of contacting a solution containing Factor VIII with a Factor VIII-binding substrate under conditions sufficient to bind Factor VIII to the substrate, wherein the Factor VIII-binding substrate comprises one or more peptides bound to the substrate, wherein the peptides are selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, and then eluting the bound Factor VIII.

REFERENCES:
patent: 5133866 (1992-07-01), Kauvar
patent: 5834318 (1998-11-01), Buettner

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