Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1992-04-16
1994-03-01
Moezie, F. T.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530415, 530416, 530417, 530324, 530399, 930120, C07K 300, C07K 1300
Patent
active
052909204
ABSTRACT:
Human epidermal growth factor is provided in an ultrapure form characterized by the absence of protein contaminants detectable by capillary electrophoresis analysis. A method for obtaining such ultrapure human epidermal growth factor includes fractionation of a human epidermal growth factor preparation by reversed phase high performance liquid chromatography in the presence of a cationic ion-pairing agent.
REFERENCES:
patent: 4717717 (1988-01-01), Finkenaur
Nishimura et al, "Heterogeneity Of Human Epidermal Growth Factor/Urogastrone From Human Urine," Chem. Pharm. Bull., 33(9):4037-4040, (1985).
O'Hare et al, "The Optimization Of RP-HPLC Of Proteins With Large Pore-Size Short Alkylchain-Bonded Silica (Ultrapore RPSC) And Its Application To Epidermal Growth Factor," Protides Biological Fluids, 30:723-726.
Gordon et al, "Capillary Electrophoresis," Science, 242:224-228, (1988).
Haigler et al, "Visualization By Fluorescene Of The Binding And Internalization Of Epidermal Growth Factor In Human Carcinoma Cells A-431," Proc. Natl. Acad. Sci. USA l , 75:3317-3321, (1978).
Hancock, "CRC Handbook Of HPLC For The Separation Of Amino Acids, Pepties, And Proteins," CRC Press, Inc., vol. 1 Table of contents only.
Sofer et al, "Designing An Optimal Chromatographic Purification Scheme For Proteins," Biotechniques, pp. 198-203, (1983).
Burgoyne et al, "High Performance Preparative Scale Purification Of Human epidermal Growth Factor," Analusis, 15(8):48-53 (1987).
Okumura et al, "Improvement In Wound Healing By Epidermal Growth Factor (EGF) Ointment. I. Effect of Nafamostat, Gabexate, Or Gelatin On Stabilization And Efficay Of EGF", Pharmaceutical Research, 7:1289-1293, 1990.
Dwivedi Chandra M.
Sindrey Dennis R.
Allelix Biopharmaceuticals Inc.
Moezie F. T.
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