Method of purifying Factor VIII:C

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530413, 530417, 424101, C07K 320, A61K 3514, A61K 3702

Patent

active

047586575

ABSTRACT:
Disclosed is a process for purifying Factor VIII:C in high yield and potency from source material containing Factor VIII:C, Factor VIII:R and other plasma proteins and factors comprising the steps of: adsorbing Factor VIII:C onto a hydrophobic interaction matrix, separating unwanted proteins from Factor VIII:C and eluting Factor VIII:C by a solution containing a surface active agent.

REFERENCES:
patent: 4069216 (1978-01-01), Shanbrom
patent: 4314997 (1982-02-01), Shanbrom
patent: 4361509 (1982-11-01), Zimmerman
patent: 4673733 (1987-06-01), Chandra et al.
Morgenthaler, Thromb. Haemostas., 47(2), 124-127, 1982.

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