Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1992-09-02
1994-06-07
Hill, Jr., Robert J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530350, 435 691, C07K 302
Patent
active
053190734
ABSTRACT:
An unconventional approach to purifying CCK receptor protein to sequenceable-grade homogeneity has been discovered. By this approach, CCK receptor protein can be obtained and sequenced routinely from a variety sources, and from the sequence information thus obtained it is possible to prepare oligonucleotides suitable for cloning CCK receptor genes. "CCK receptor" in this context denotes any from a group of proteins that displays a characteristic CCK binding affinity and that is encoded by a nucleotide sequence which hybridizes a oligonucleotide probe designed in accordance with the criteria elaborated herein.
REFERENCES:
Meth. in Ehly, 104: 97-113 (1984) Lowe et al., Affinity Chromatography on Immobilized Dyes.
A. A. Dufresne, et al., "Purification of A-subtype pancreatic cholecystokin receptor by immunoaffinity chromatography", Biochimie (1992) 74, 149-151.
A. B. Wank, et al., "Brain and gastrointestinal cholecystokinin receptor family: Structure and functional expression", Proc. Natl. Acad. Sci., U.S.A., vol. 89, pp. 8691-8695, Sep. 1992.
A. C. Pisegna, et al., "Molecular Cloning of the Human Brain and Gastric Cholecystokinin Receptor: Structure, Functional Expression and Chromosomal Localization", Biochemical and Biophysical Research Communications, vol. 189, No. 1, Nov. 30, 1992, pp. 296-303.
Kopin et al., "Expression cloning anc characterization of the canine parietal cell gastrin receptor", PNAS USA 89:3605 (1992).
Duong et al., "Purification and Characterization of the Rat Pancreatic Cholecystokinin Receptor", J. Biol. Chem. 264(30): 17990 (1989).
Szecowka et al., "Purification of the pancreatic cholecystokinin receptor", Regulatory Peptides 24:215 (1989).
Knapp et al., "A New, Highly Selective CCK-B Receptor Radioligand ([.sup.3 H][N-methyl-Nle.sup.28,31 ]CCK.sub.26-33):Evidence for CCK-B Receptor Heterogeneity", J. Pharm. Exp. Ther. 255(3): 1278 (1990).
Grider et al., "Distinct receptors for cholecystokinin and gastrin on muscle cells of stomach and gallbladder", Gastrointest. Liver Physiol. 22: G184 (1990).
Roche et al.l, "Characterization of a gastrin-type receptor on rabbit gastric parietal cells using L365, 260 an L364, 718", Gastrointest. Liver Physiol. 23: G182 (1991).
Wank et al., "Purification, molecular cloning, and functional expression of the cholecystokinin receptor from rat pancreas", PNAS USA 89: 3125 (Apr., 1992).
Roche et al., "Gastrin and CCK" Receptors on Histamine-and Somatostatin-Containing Cells from Rabbit Fundic Mucosa-II, Biochem. Pharmacol. 42(4): 771 (1991).
Chemer et al., "Functionally distinct receptors for cholecystokinin and gastrin on dispersed chief cells from guinea pig stomach", Gastrointest. Liver Physiol. 17: G151 (1988).
Hill Jr. Robert J.
The United States of America as represented by the Department o
Ulm John D.
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