Method of purifying calcium ion-binding protein

Details Method of purifying calcium ion-binding protein Method of purifying calcium ion-binding protein

2005-03-22

2005-03-22

Campbell, Bruce R. (Department: 1654)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C514S002600, C514S020800, C530S350000, C530S417000, C530S384000, C530S413000, C530S380000, C530S425000, C530S381000

Reexamination Certificate

active

06869934

ABSTRACT:
The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.

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