Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-11-14
1997-09-09
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 2, 435 30, 435378, 435372, C12N 508, C12Q 124
Patent
active
056655576
ABSTRACT:
The invention relates to methods of obtaining compositions enriched in hematopoietic stem cells by separating out an enriched fraction of cells expressing the marker CDw109. Methods of obtaining compositions enriched in hematopoietic megakaryocyte progenitor cells are also provided. Compositions enriched for stem cells and populations of cells obtained therefrom are also provided by the invention. Methods of use of the cells are also included.
REFERENCES:
patent: 4714680 (1987-12-01), Civin
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5147784 (1992-09-01), Peault
patent: 5464753 (1995-11-01), Chaudhary et al.
Baum et al. "Isolation of a candidate human hematopoietic stem-cell population" Proc. Natl. Acad. Sci. USA (1992) 89:2804-2808.
Spangrude et al. "Purification and characterization of mouse hematopoietic stem cells" Science (1988) 241:58-62.
Smith et al. "Clonal analysis of hematopoietic stem-cell differentiation in vivo" Proc. Natl. Acad. Sci. USA (1991) 88:2788-2792.
Uchida, Dialog.TM. Dissertation Abstract Ph.D. Thesis (1992) Stanford University, 2 pages total.
Sutherland et al., "Identification of a cell-surface antigen associated with activated T lymphoblasts and activated platelets" Blood (1991) 77:84-93.
DiGiusto et al., "Human fetal bone marrow early progenitors for T, B, and myeloid cells are found exclusively in the population expressing high levels of CD34" Blood (1994) 84:421-432.
Whitlock et al., "Long-term culture of B lymphocytes and their precursors from murine bone marrow" Proc. Natl. Acad. Sci. USA (1982) 79:3608-3612.
Whitlock et al., "Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule" Cell (1987) 48:1009-1021.
Spangrude, "Enrichment of murine haemopoietic stem cells: diverging roads" Immunol. Today (1989) 10:344-350.
Haugland, Handbook of Fluorescent Probes and Research Chemicals, Larison, K.D., ed., 5th ed., Molecular Probes, Inc., (1992). The title page and table of contents are included herewith.
Kyoizumi et al., "Implantation and maintenance of functional human bone marrow in SCID-hu mice" Blood (1992) 79:1704-1711.
Metcalf, Hemopoietic Colonies. In vitro Cloning of Normal and Leukemic Cells, (1977) Springer-Verlag, Germany. The title page and table of contents are included herewith.
Sambrook et al., Molecular Cloning A Laboratory Manual, 2nd ed., (1989) Cold Spring Harbor Laboratory Press, New York. The title page and table of contents are included herewith.
Marsh et al., "Retention of progenitor cell function in CD34.sup.+ cells purified using a novel O-sialoglycoprotease" Leukemia (1992) 6:926-934.
Brandt et al., "Role of c-kit ligand in the expansion of human hematopoietic progenitor cells" Blood (1992) 79:634-641.
Bruno et al., "Effect of recombinant and purified hematopoietic growth factors on human megakaryocyte colony formation" Exp. Hematol. (1988) 16:371-377.
Bruno et al., "Interacting cytokines regulate in vitro human megakaryocytopoiesis" Blood (1989) 73:671-677.
Briddell et al., "Characterization of the human burst-forming unit-megakaryocyte" Blood (1989) 74:145-151.
de Sauvage et al., "Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand" Nature (1994) 369:533-538.
Kaushansky et al., "Promotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombopoietin" Nature (1994) 369:568-571.
Wendling et al., "c-Mpl ligand is a humoral regulator of megakaryocytopoiesis" Nature (1994) 369:571-574.
Bartley et al., "Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl" Cell (1994) 77:1117-1124.
Lefkovits et al., "Limiting dilution analysis of the cells of immune system. I. The clonal basis of the immune response" Immunol. Today (1984) 5:265-268.
Schneider et al., "A one-step purification of membrane proteins using a high efficiency immunomatrix" J. Biol. Chem. (1982) 257:10766-10769.
Sutherland et al., "Sensitive detection and enumeration of CD34.sup.+ cells in peripheral and cord blood by flow cytometry" Exp. Hematol. (1994) 22:1003-1010.
Yeo et al "CDw109: A Novel Platelet and Endothelial Cell Actuation Specific Antigen", Abstract No. 19 Published in Conjunction with 1994 Canadian Red Cross Scientific Meeting, Chateau Whistler, British Columbia (Apr. 23-26, 1994).
Yeo, et al. "A Benard-Soulier-Like Defect: Selective Cleavage of GPb.alpha. with a Novel O-Sialoglycoprotein Endopeptidase" Abstract No. 31 published in conjuction with 1994 Canadian Red Cross Scientific Meeting, Chateau Whistler, British Columbia (Apr. 23-26, 1994).
Farley et al. "Divergem Molecular Phenotypes of KG1 and KG1 a Myeloid Cell Lines" (1986) Blood Vol 68(5):1101-1107.
Murray Lesley
Sutherland D. Robert
Saunders David
Systemix, Inc.
The Toronto Hospital Research Institute
LandOfFree
Method of purifying a population of cells enriched for hematopoi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of purifying a population of cells enriched for hematopoi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of purifying a population of cells enriched for hematopoi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-68821