Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Patent
1995-03-07
1998-11-17
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
4352351, 435 691, 4353201, 435803, C12N 702, C12N 700, C12N 1500
Patent
active
058375201
ABSTRACT:
The invention provides a method for purifying viral vectors containing therapeutic genes for use in gene therapy. The invention comprises a method of purification from a cell lysate of a recombinant viral vector containing a therapeutic gene, which comprises: a) treating said lysate with an enzymatic agent that selectively degrades both unencapsulated DNA and RNA; b) chromatographing the treated lysate from step a) on a first resin; and c) chromatographing the eluant from step b) on a second resin; wherein one resin is an anion exchange resin and the other is an immobilized metal ion chromatography (IMAC) resin.
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Huyghe Bernard G.
Liu Xiaodong
Shabram Paul W.
Shepard H. Michael
Canji Inc.
Chambers Jasemine C.
Hauda Karen M.
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