Method of purification of thermally stable enzymes

Details Method of purification of thermally stable enzymes Method of purification of thermally stable enzymes

1979-11-13

1981-02-10

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435814, 435827, 435853, 435886, C12N 992

Patent

active

042502632

ABSTRACT:
A composition comprising intracellular or extracellular glucose isomerase may be purified by a method comprising heat treatment at a temperature from about 40.degree. C. to about 80.degree. C. The resultant enzyme solution, when utilized to prepare an immobilized enzyme system, is operationally equivalent to glucose isomerase purified by the traditional physico-chemical methods.

REFERENCES:
patent: 3948728 (1976-04-01), Roeschlau et al.
Yamanaka, "D-Xylose Isomerase" in Methods in Enzymology, vol. IX, pp. 588-593 (1966).

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