Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1979-03-12
1980-05-27
Adee, John
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
210 31C, 435815, B01D 1508
Patent
active
042051304
ABSTRACT:
A new method of purification of human prostatic acid phosphatase has been provided. The starting point for the purification process is constituted by a solution containing acid phosphatase and having its origin in a sample derived from a human body. If required, said sample is subjected to a pretreatment so as to draw the phosphatase into solution and to remove any solid tissues. The solution is then treated by means of affinity chromatography, whereby the phosphatase in question is separated from other proteins. The chromatographic step is performed in a column packed with an agarose gel coupled with tartrate groups. A sodium acetate buffer containing tartrate may be used as an eluent and the eluate is collected as successive fractions. Those fractions containing the phosphatase are further treated by means of isoelectric focusing so as to separate the different isoenzymes of the phosphatase from each other. The isoelectric focusing may be performed either in a column or on a disc covered with a gel.
REFERENCES:
patent: 3901763 (1975-08-01), Horiuchi et al.
patent: 3947352 (1976-03-01), Cuatrecasas et al.
patent: 4166766 (1979-09-01), Metzenberg et al.
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