Method of purification of glucose isomerase

Details Method of purification of glucose isomerase Method of purification of glucose isomerase

1979-11-13

1981-03-17

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435814, 435815, 435827, 435853, 435886, C12N 992

Patent

active

042568382

ABSTRACT:
A process for purifying an enzyme such as glucose isomerase which comprises precipitating nucleic acids from a cell-free, heat-treated enzyme solution in a suitable buffer and chromatographing the supernatant on a cellulosic medium. Subsequent chromatography on a hydrophilic, molecular-sieve medium affords enzyme of about 90% purity.

REFERENCES:
patent: 4077842 (1978-03-01), Cory
Palleroni et al., "Mannose Isomerase of Pseudomonas Saccharophila", Journal of Biological Chemistry, 1956, vol. 218, pp. 535-548.
Yamanaka, "D-Xylose Isomerase", Methods in Enzymology, vol. IX, pp. 588-593, (1966).
Takasaki et al., "Studies on Sugar Isomerizing Enzyme", Agr. Biol. Chem., vol. 33, No. 11, pp. 1527-1534, (1969).

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