Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-03-31
1997-03-11
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 436533, G01N 33554, G01N 33569
Patent
active
056100230
ABSTRACT:
A method is provided for the purification of Clostridium difficile Toxin A comprising reacting impure Toxin A with immobilized mono-specific polyclonal antibodies. The polyclonal antibodies are coupled to a hydrazide group containing matrix such as hydrazide activated agarose gel. The immobilized antibody is specific for Toxin A and will greatly purify Toxin A from a Toxin A containing solution. Antibodies raised to Toxin A purified according to the method are of higher activity than antibodies produced from prior art purified Toxin A.
REFERENCES:
patent: 4530833 (1985-07-01), Wilkins et al.
patent: 4533630 (1985-08-01), Wilkins et al.
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4689299 (1987-08-01), Insel et al.
patent: 4863852 (1989-09-01), Wilkins et al.
patent: 4879218 (1989-11-01), Wilkins et al.
patent: 4937201 (1990-06-01), Ueno et al.
patent: 4959457 (1990-09-01), Bringman
patent: 5071759 (1991-12-01), Rothman et al.
patent: 5098826 (1992-03-01), Wilkins et al.
patent: 5231003 (1993-07-01), Coughlin et al.
patent: 5266315 (1993-11-01), Taguchi et al.
patent: 5316911 (1994-05-01), Baek et al.
patent: 5545721 (1996-08-01), Carroll et al.
Lyerly et al., Monoclonal and Specific Polyclonal Antibodies for Immunoassay of C. difficile Toxin A, Jrnl. of Clinical Microbiology, Jan. 1985, p. 12-14.
Domen et al., Site-directed immobilization of proteins, Jrnl. of Chromatography, 510 (1990) 293-302.
Ehrich et al., Production of C. difficile Antitoxin, Infection and Immunity, Jun. 1980, pp. 1041-1043.
Robert K. Scopes, Separation by Precipitation, Protein Purification (2d edition) pp. 40-45.
Muller et al., Monoclonal Antibodies Specific for C. difficile Toxin B and Their Use in Immunoassays, Jrnl. of Clinical Microbiology, Jun. 1992, pp. 1544-1550.
DiPersio et al., Development of a Rapid Enzyme Immunoassay for C. difficile Toxin A and Its Use in the . . . Disease, Jrnl. of Clinical Micro., Dec. 1991, pp. 2724-2730.
Ryan et al., Rapid Detection of C. difficile Toxin in Human Feces, Jrnl. of Clinical Microbiology, Dec. 1980, pp. 776-779.
Warny et al., Human Antibody Response to C. difficile Toxin A in Relation to Clinical Course of Infection, Infection And Immunity, Feb. 1994, pp. 384-389.
Wolfhagen et al., Toxins A and B of C. difficile, FEMS Microbiology Reviews 13 (1994) 59-64.
O'Shannessy et al., Immobilization of Glycoconjugates by Oligosaccharides: Use of Hydrazido-Derivatized Matrices, Analytical Biochemistry 191, 1-8 (1990).
Lyerly et al., Enzyme-Linked Immunosorbent Assa for C. difficile Toxin A, Jrnl. of Clinical Microbiology, Jan. 1983, pp. 72-78.
Pierce Ordering Catalog, pp. 3-10, (1989).
Domen et al.; Site-Directed Immobilization of Proteins; Jrnl. of Chromatography, 510 (1990) 293-302.
Depitre et al.; Detection of Clostridium difficile Toxins in Stools; Gastroenterol Clin Biol, 1993, 17, 283-286.
Kamiya et al.; Production of Monoclonal Antibody to Clostridium difficile Toxin A . . . Activity; FEMS Microbiology Letters (1991) 311-316.
Katoh et al.; Purification and Some Properties of Cytotoxin Produced by Clostridium difficile; Microbiol. Immunol.; vol. 32 (6), 551-564, 1988.
Ascione et al.; Accuracy in Detection of Clostridium difficile Toxin Direct . . . Colitis; Current Therapeutic Research; vol. 36, No. 5, Nov. 1984.
Grace M. Thorne; Diagnostic Tests in Gastrointestinal Infections; 1994 Current Science ISBN 1-85922-606-X ISSN 0267-1379.
Antibodies, A Laboratory Manual; Harlow, E. et al, Cold Spring Harbor Laboratory; 1988.
Detection of Clostridium difficile toxins in stools.; Depitre, C. et al Gastroenterol Clin. Biol (France) 1993 17(4) pp. 283-286.
Accuracy in detection of Clostridium difficile toxin; Ascione, A; Current Therapeutic Research, vol. 36 #5, Nov. 84, pp. 882-892.
Tresyl activation of a hydroxyalkyl ligand for coupling a hydrazide gel: stable immobilization of T-2 toxin for affinity purification of T-2 antibody; Allam, K; 1987 Anal. Biochem; vol. 162, #1, pp. 171-177.
Diagnostic tests in gastrointestinal infections, Thorne, G. M. 1994, Curr. Opin Gastroenterol vol. 10, 1 (112-118).
Purification and some properties of cytotoxin produced by Clostridium difficile; Katoh. T, Microbiol. Immunol, 1988 32/6 (551-564).
Production of monoclonal antibody to Clostridium difficile toxin A which neutralizes enterotoxicity but not hemagglutination activity; (1991), vol. 81, #3 pp. 311-315, Fems. Microbiol. Lett.
Housel James C.
Lee Laboratories, Inc.
Portner Ginny Allen
LandOfFree
Method of purification of clostridium difficile toxin A and prod does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of purification of clostridium difficile toxin A and prod, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of purification of clostridium difficile toxin A and prod will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-442479