Method of purification of cellulose from a broth solution by cry

Details Method of purification of cellulose from a broth solution by cry Method of purification of cellulose from a broth solution by cry

1998-08-28

2000-07-11

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435816, 117927, C12N 942, C30B 2958

Patent

active

060871482

ABSTRACT:
A method for purification, and isolation in crystalline form, of a cellulase from a broth comprises: treating the broth with a crystallization-effective amount of a water-miscible organic solvent (e.g. a lower aliphatic alcohol or ketone); and isolating the cellulase in question in crystalline form.

REFERENCES:
patent: 3664926 (1972-05-01), Grabner et al.
patent: 5763254 (1998-06-01), Woldike et al.
Joliff et al., J. Mol. biol., "Crystallization and Preliminary X-ray Diffraction Study of an Endoglucanase from Clostridium thermocellum", 189(1), p. 249-250, May 1986.
Joliff et al., Bio/Technology, "Isolation, Crystallization and Properties of a New Cellulase of Clostridium themocellum Overproduced in Escherichia coli", 4, pp. 896-900, Oct. 1986.
Davies et al., J. Mol. Biol., "Crystallization and Preliminary X-ray Analysis of a Fungal Endoglucanase I", 2228(3), pp. 970-972, Dec. 1992.
Spezio et al., Biochemistry, "Crystal Structure of the Catalytic Domain of a Thermophilic Endocellulase", 32(38), pp. 9906-9916, Sep. 1993.
Davies e al., Nature, "Structure and Function of Endoglucanse V", 365(6444), pp. 362-364, Sep. 1993.
Hata et al., J. Mol. Biol., "Crystallization and Preliminary X-ray Diffraction Studies of an Endoglucanase from Aspergillus aculeatus", 241 (2), pp. 278-280, Aug. 1994.
Chauvaux et al., J. Biol. Chem., "Structural and Functional Analysis of the Metal-Binding Sites of Clostridium thermocellum Endoglucanase CelD", 270(17), pp. 9757-9762, Apr. 1995.
Chitarra et al., J. Mol. Biol., "Multiple Crystal Forms of Endoglucanase CelD: Signal Peptide Residucs Modulate Lattice Formation", 218(2), pp. 225-232, Apr. 1995
Hahn et al., FEBS Letts., "Crystal Structure of Bacillus licheniformis 1,3-1,4-beta-D-Glucan 4-Glucanohydrolase at 1.8 Angstrom Resolution", 374(2), pp. 221-224, Aug. 1995.
Souchon et al., Prot. Struct. Funct. Genet., "Crystallization of a Family 8 Cellulase from Clostridium thermocellum", 25(1), pp. 134-136, May 1996.
Dounce et al. Prep. Biochem. (1981) 11(5): 501-523 (abstract only).
McPherson, A., Method in Enzomol. (1985) 114:112-120.
Scopes, R.K. "Protein Purification: Principles and Practice," 2nd edition, (1987) (Springer-Verlag: New York) p. 297-301.
Minoda et al. Agr. Biol. Chem. (1963) 27(11):806-11.
Kubo, Y. Toyama-ken Yakuji Kenkyusho Nenpo (1994) 21(21): 68-72 (abstract only).
McPherson, Eur. J. Biochem. (1990) "Protein Purification by Bulk Crystallization: The Recovery of Ovalbumin" 189:1-23.
Judge et al. (1995) Biotechnology and Bioengineering "Current Approaches To Macromolecular Crystallization" 48:316-323.

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