Method of purification of carboxypeptidase G.sub.1

Details Method of purification of carboxypeptidase G.sub.1 Method of purification of carboxypeptidase G.sub.1

1976-06-18

1978-03-14

Shapiro, Lionel M.

Textiles: fiber preparation

C07G 7028

Patent

active

040789721

ABSTRACT:
A method of purifying a protein-reaction mixture to obtain carboxypeptidase G.sub.1, which method comprises: passing the mixture through a column containing support material, such as carboxymethyl cellulose, under pH conditions such that the carboxypeptidase G.sub.1 is preferentially and selectively bonded to active sites on the support material in preference to other protein materials of the mixture; displacing the bound carboxypeptidase G.sub.1 by an eluant, such as a glutamate, which has a greater affinity for the carboxypeptidase G.sub.1 than the support material; and recovering from the column a mixture of the eluant and the carboxypeptidase G.sub.1.

REFERENCES:
mcCullough et al., Journal of Biological Chemistry, vol. 246, No. 10, pp. 7207-7213 (1971).
Uren, Biochimica et Biophysica Acta, vol. 236, (1971), pp. 67-73.

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