Method of purification of amylase by precipitation with a metal

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435201, C12N 926, C12N 928

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active

052815269

ABSTRACT:
The present invention relates to a method for the preparation of a purified amylase recovered from a fermentation broth, by the addition of a precipitation agent which is a halide salt, most preferably sodium or potassium chloride. According to a preferred embodiment, a further precipitation agent is added which is an organic compound, most preferably an alkyl ester of 4-hydroxybenzoic acid.
The present invention also relates to the purified amylases so obtained and to the uses of these purified amylases.

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patent: 4717662 (1988-01-01), Montgomery et al.
patent: 5041377 (1991-08-01), Becker et al.
Horikoshi et al. A New Microbial World, Springer-Verlag, M.Y. pp. 93-143 (1981).
Starace et al. Encyclopedia of Chemical Technology, 9, 138-148 (1980).

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