Method of proteolytically cleaving prothrombin to produce thromb

Details Method of proteolytically cleaving prothrombin to produce thromb Method of proteolytically cleaving prothrombin to produce thromb

1993-03-30

1995-07-11

Knode, Marian C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435214, 424 9464, C12P 2106, C12N 974, A61K 3243

Patent

active

054320623

ABSTRACT:
Prothrombin is cleaved by proteases in the presence of a detergent or certain chaotropic substances to produce thrombin. Under these conditions, controlled and restricted proteolysis occurs such that significant activation without digestion occurs. The chaotropic substances may be urea, guanidinium hydrochloride or a thiocyanate salt. Reversible immobilization of prothrombin on a solid support prior to activation improves the selectivity of the proteolytic activation.

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