Method of protein production using mitochondrial translation sys

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 691, 435 701, 435 703, C12N 1509

Patent

active

06100068&

ABSTRACT:
A method of producing viral antigens in vitro by infecting animal organ tissue rich in mitochondria with a virus, including human hepatitis B virus (HBV), and culturing the infected tissue in vitro is disclosed. A method of producing proteins in vitro by transfecting mitochondria-rich animal tissue with a recombinant HBV-based vector and culturing the transfected tissue in a dynamic tissue culture system is disclosed.

REFERENCES:
Zuckerman et al. The American Journal of the Medical Sciences. vol. 270(1), pp. 197-204, 1975.
Cheng et al. Journal of Virology. vol. 61(4), pp. 1286-1290, Apr. 1987.
Belyaev et al. Doklady Akademi Nauk SSSR, vol. 314(2), pp. 488-491 (translation), Sep. 1990.
Alberts, Bruce, et al., Molecular Biology of the Cell, Second Edition, Garland Publishing, Inc., New York & London, Chapter 7, pp. 387-401, 1989.
Jensen, T.G., et al., Biochimica et Biophysica Acta, 1180: 65-72, 1992, "Expression of wild-typ and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells."
Araki, Kimi, et al., Proc. Natl., Acad. Sci. USA, 86: 207-211, Jan. 1989, "Expression and replication of hepatitis B virus genome in transgenic mice."
Chang, Ghien-Neng, et al., The Journal of Biological Chemistry, 267(31): 22414-22420, Nov. 5, 1992, "Biochemical Pharmacology of (+)--and (-)--2', 3'--Dideoxy-3'-thiacytidine as Anti-hepatitis B Virus Agents."
Chisari, Francis V., Hepatology, 22(4): 1316-1325, 1995, "Hepatitis B Virus Transgenic Mice: Insights Into the Virus and the Disease."
Colacino, J.M., S.K. Malcolm, and S.R. Jaskunas, Antimicrobial Agents and Chemotherapy, 38(9): 1997-2002, Sep. 1994, "Effect of Fialuridine on Replication of Mitochondrial DNA in CEM Cells and in Human Hepatoblastoma Cells in Culture."
Doong, Shin-Lian, et al., Proc. Natl. Acad. Sci. USA, 88: 8495-8499, Oct. 1991, "Inhibition of the replication of hepatitis B virus in vitro by 2', 3'-dideoxy-3'-thiacytidine and related analogues."
Elfassi, Emile, et al., Proc. Natl. Acad. Sci. USA, 81: 3526-3528, Jun. 1984, "Evidence of extrchromosoma forms of hepatitis B viral DNA in a bone marrow culture obtained from a patient recently infected with hepatitis B virus."
Feray, C., et al., Transplantation, 49(6): 1155-1158, Jun. 1990, "Persistent Hepatitis B Virus Infection of Mononuclear Blood Cells without Concomitant Liver Infection."
Cuidotti, Luca G., Brent Matzke, Heinz Schaller, and Francis Chisari, Journal of Virology, 69(10): 6158-6169, Oct. 1995, "High-Level Hepatitis B Virus Replication in Transgenic Mice."
Gripon, Phillippe, Christian Diot, and Christiane Gugen-Guillouzo, Virology, 192: 534-540, 1993, "Reproducible High Level Infection of Cultured Adult Human Hepatocytes by Hepatitis B Virus: Effect of Polyethylene Gycol on Adsorption and Penetration."
Kato, Nobuyuki, et al., Proc. Natl. Acad. Sci. USA, 87: 9524-9528, Dec. 1990, "Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis."
Lau, Johnson, Y.N. and Teresa L. Wright, The Lancet, 342:1335-1340, Nov. 17, 1993, "Molecular virology and pathogenesis of hepatitis B."
Ochiya, Takahiro, et al., Proc. Natl. Acad. Sci. USA, 86: 1875-1879, Mar. 1989, "An in vitro system for infection with hepatitis B virus that uses primary human fetal hepatocytes."
Okaomoto, Hiroaki and Shunji Mishiro, Intervirology, 37: 68-76, 1994, "Genetic Heterogeneity of Hepatitis C Virus."
Paik, K-H, et al., Hepatology, 22(4), Pt. 2, AASLD Abstracts, Abstract 1459, 1995, "The Mitochondron is the Key Organelle for Hepatitis B Virus Infection."
Peranen, Johan, Journal of General Virology, 72: 195-199, 1991, "Localization and phosphorylation of Semliki Forest virus non-structureal protein nsP3 expressed in COS cells from a cloned cDNA."
Shavrina Asher, Ludmila V, Leonard N. Binn, and Ruth H. Marchwicki, Journal of Virology Methods, 15: 323-328, 1987. "Demonstration of hepatitis A virus in a cell culture by electron microscopy with immunoperoxidase staining."
Shimizu, Yohko, et al., Proc. Natl. Acad. Sci. USA, 89: 5477-5481, Jun. 1992, "Evidence for in vitro replicationof hepatitis C virus genome in a human T-cell line."
Smith, P.F., et al., Life Sciences, 36: 1367-1375, Nov. 14, 1985, "Dynamic Organ Culture of Precision Liver Slices for In Vitro Toxicology."
Yang, Young-II, and Kye-Hyung Paik, Manuscript, not yet submitted for publication, "Replication of Human Hepatitis B Virus (HBV) Genome in Rats Evidenced by Covalently Closed Circular DNA (CCC DNA)."

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of protein production using mitochondrial translation sys does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of protein production using mitochondrial translation sys, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of protein production using mitochondrial translation sys will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1149087

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.