Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2001-06-28
2004-11-30
Brumback, Brenda (Department: 1647)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S325000, C435S375000, C530S350000, C530S351000, C530S399000
Reexamination Certificate
active
06824973
ABSTRACT:
2. TECHNICAL FIELD
The present invention provides novel polynucleotides and proteins encoded by such polynucleotides, along with uses for these polynucleotides and proteins, for example in therapeutic, diagnostic and research methods. In particular, the invention relates to a novel human stem cell growth factor-like protein.
2.1 Background Art
Technology aimed at the discovery of protein factors (including e.g., cytokines, such as lymphokines, interferons, circulating soluble factors, chemokines, and interleukins) has matured rapidly over the past decade. The now routine hybridization cloning and expression cloning techniques clone novel polynucleotides “directly” in the sense that they rely on information directly related to the discovered protein (i.e., partial DNA/amino acid sequence of the protein in the case of hybridization cloning; activity of the protein in the case of expression cloning). More recent “indirect” cloning techniques such as signal sequence cloning, which isolates DNA sequences based on the presence of a now well-recognized secretory leader sequence motif, as well as various PCR-based or low stringency hybridization-based cloning techniques, have advanced the state of the art by making available large numbers of DNA/amino acid sequences for proteins that are known to have biological activity, for example, by virtue of their secreted nature in the case of leader sequence cloning, by virtue of their cell or tissue source in the case of PCR-based techniques, or by virtue of structural similarity to other genes of known biological activity.
Identified polynucleotide and polypeptide sequences have numerous applications in, for example, diagnostics, forensics, gene mapping; identification of mutations responsible for genetic disorders or other traits, to assess biodiversity, and to produce many other types of data and products dependent on DNA and amino acid sequences.
3. SUMMARY OF THE INVENTION
The present invention provides an isolated polynucleotide encoding a polypeptide having stem cell growth factor activity, said polynucleotide comprising the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 or the mature protein coding portion thereof, or a fragment, analog, variant or derivative thereof that encodes a polypeptide retaining stem cell growth factor activity. These polypeptides include those which hybridize to the complement of the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 under stringent hybridization conditions, those which comprise a nucleotide sequence having greater than about 85% sequence identity with the nucleotide sequence of SEQ ID NO:9, 11, 12, 31 or 33, those which comprise a nucleotide sequence having greater than about 90% sequence identity with the nucleotide sequence of SEQ ID NO:9, 11, 12, 31 or 33 and those polypeptides which comprise a nucleotide sequence having greater than about 92% sequence identity with the nucleotide sequence of SEQ ID NO:9, 11, 12, 31 or 33. 14. The polynucleotides may be a DNA. The present invention also encompasses polynucleotides which comprise the complement of these polynucleotides.
The present invention provides for isolated polynucleotide encoding a polypeptide having stem cell growth factor activity, said polynucleotide comprising the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 or the mature protein coding portion thereof, or a fragment, analog, variant or derivative thereof that encodes a polypeptide retaining stem cell growth factor activity with the proviso that said polynucleotide sequence does not consist of the nucleotide sequence of SEQ ID NO: 47. These polypeptides include those which hybridize to the complement of the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 under stringent hybridization conditions and with the proviso that said polynucleotide sequence does not consist of the nucleotide sequence of SEQ ID NO: 47, those which comprise a nucleotide sequence having greater than about 85% sequence identity with the nucleotide sequence of SEQ ID NO:9, 11, 12, 31 or 33 and with the proviso that said polynucleotide sequence does consist of the nucleotide sequence of SEQ ID NO: 47, those which comprise a nucleotide sequence having greater than about 90% sequence identity with the nucleotide sequence of SEQ ID NO:9, 11, 12, 31 or 33 and with the proviso that said polynucleotide sequence does not consist of the nucleotide sequence of SEQ ID NO: 47, and those polypeptides which comprise a nucleotide sequence having greater than about 92% sequence identity with the nucleotide sequence of SEQ ID NO:9, 11, 12, 31 or 33 and with the proviso that said polynucleotide sequence does not consist of the nucleotide sequence of SEQ ID NO: 47.
The present invention provides for an isolated polynucleotide that comprises the mature protein coding sequence of SEQ ID NO: 9, 11, 12,31 or 33. The invention also provides for an isolated polynucleotide that comprises the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33.
The invention provides for a DNA encoding a polypeptide having stem cell growth factor activity, said polynucleotide comprising the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 or the mature protein coding portion thereof, or a fragment, analog, variant or derivative thereof that encodes a polypeptide retaining stem cell growth factor activity wherein the encoded polypeptide has an amino acid sequence comprising at least amino acid residues 22 to 279 of SEQ ID NO: 32, or an amino acid sequence comprising at least amino acid residues 22 to 272 of SEQ ID NO: 34; or the encoded polypeptide has an amino acid sequence including deletion, substitution or insertion of one or several amino acids in the amino acid sequence comprising at least amino acid residues 22 to 279 of SEQ ID NO: 32, or an amino acid sequence comprising at least amino acid residues 22 to 272 of SEQ ID NO: 34, and which has an activity to support proliferation or survival of hermatopoietic stem cell or hematopoietic progenitor cell, with a proviso that C-terminal amino acid sequence does not comprise the amino acid sequence of SEQ ID NO: 46.
The invention provides for a DNA encoding comprising the nucleotide stem cell growth factor activity, said polynucleotide comprising the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 or the mature protein coding portion thereof, or a fragment, analog, variant or derivative thereof that encodes a polypeptide retaining stem cell growth factor activity, which is a DNA which comprises at least nucleotides 574 to 1347 of SEQ ID NO: 31; or a DNA which is hybridizable with the nucleotide sequence of SEQ ID NO: 31 or a probe prepared from said sequence, under stringent conditions, and which has an activity to support proliferation or survival of hematopoietic stem cell or hematopoietic progenitor cell. These include DNAs which hybridize under the following stringent conditions: 6×SSC,5×Denhardt, 0.5% SDS and 68° C. (SSC 3M NaCl, 0.3M sodium citrate, 50×Denhardt/1% BSA/1% polyvinyl pyrrolidone, 1% Ficoll 400/, or 6×SSC, 5×Denhardt, 0.5% SDS, 50% formamide and 42° C.
The invention provides for a DNA encoding a polynucleotide encoding a polypeptide having stem cell growth factor activity, said polynucleotide comprising the nucleotide sequence of SEQ ID NO: 9, 11, 12, 31 or 33 or the mature protein coding portion thereof, or a fragment, analog, variant or derivative thereof that encodes a polypeptide retaining stem cell growth factor activity, which is a DNA which comprises at least nucleotides 321 to 1074 of SEQ ID NO: 33; or DNA which is hybridizable with the nucleotide sequence of SEQ ID NO: 33 or a prove prepared from said sequence, under stringent conditions, and which has an activity to support proliferation or survival of hematopoietic stem cell or hematopoietic progenitor cell. These include DNAs which hybridize under the following stringent conditions: 6×SSC/5×Denhardt, 0.5% SDS and 68° C. (SSC 3M NaCl, 0.3M sodium citrate, 50×Denhardt/1% BSA/1% polyvinyl pyrrolidone, 1% Ficoll 400, or 6×SSC, 5×
Chao Cheng-Chi
Drmanac Radoje T.
Labat Ivan
Mize Nancy
Nishikawa Mitsuo
Brumback Brenda
Bunner Bridget E.
Kirin Beer Kabushiki Kaisha
Marshall & Gerstein & Borun LLP
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