Method of production of poly-.beta.-hydroxyalkanoate copolymers

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4252527, 42525234, 42525235, C12P 762

Patent

active

058916865

ABSTRACT:
The present invention provides methods for the production of poly-.beta.-hydroxyalkanoate copolymer comprising the steps of (a) introducing into a prokaryotic host cell a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway, (b) introducing into the host cell a vector construct which directs the expression of one or more proteins which regulate acetate and propionate metabolism, (c) culturing the host cell in medium containing propionate or a derivative thereof, and (d) isolating poly-.beta.-hydroxyalkanoate copolymer from the cultured host cell.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4336334 (1982-06-01), Powell et al.
patent: 4396763 (1983-08-01), Tsuchiya et al.
patent: 4433053 (1984-02-01), Hughes et al.
patent: 4634678 (1987-01-01), Salstrom et al.
patent: 4743453 (1988-05-01), Ahern et al.
patent: 4760022 (1988-07-01), Molin et al.
patent: 4806471 (1989-02-01), Molin et al.
patent: 4806480 (1989-02-01), Lopez
patent: 4876331 (1989-10-01), Doi
patent: 4950749 (1990-08-01), Johal et al.
patent: 5017692 (1991-05-01), Zurawski et al.
patent: 5229279 (1993-07-01), Peoples et al.
patent: 5240837 (1993-08-01), Tomich et al.
patent: 5244806 (1993-09-01), Bang et al.
patent: 5245023 (1993-09-01), Peoples et al.
patent: 5250430 (1993-10-01), Peoples et al.
patent: 5334520 (1994-08-01), Dennis
patent: 5395927 (1995-03-01), Bock et al.
Peoples and Sinskey, "Genes to PHA Polymers," International Symposium on Biodegradable Polymers: p. 108, Oct. 29-31, 1991.
Steinbuchel and Schlegel, "Physiology and molecular genetics of poly(.beta.-hydroxyalkonoic acid) synthesis in Alcaligenes eutrophus," Molecular Microbiology 5(3); 535-542, 1991.
Fidler and Dennis, "Polyhydroxyalkanoate production in recombinant Escherichia coli," FEMS Microbiology Reviews 103: 231-236, 1992.
Jenkins and Nunn, "Regulation of the ato Operon bt the atoC Gene in Escherichia coli," Journal of Bacteriology 169(5): 2096-2102, 1987.
Johnson et al., "Induction Studies on the Poly-.beta.-Hydroxybutyrate Biosynthetic Pathway in Alcaligenes eutrophus H16 and in Excherichia coli," Virginia Journal of Science: 150, 1987.
Maloy and Nunn, "Role of Gene fadR in Escherichia coli Acetate Metabolism," Journal of Bacteriology 148(1): 83-90, 1981.
Slater and Dennis, "Cloning and Expression of the Alcaligenes eutrophus H16 Beta Ketothiolase Gene in Escherichia coli," American Society for Microbiology, Annual Meeting, Abst. #H-123, Mar. 1-6, 1987.
Slater et al., "Cloning of the Alcaligenes eutrophus H16 Poly-.beta.-Hydroxybutyrate Biosynthetic Pathway into Escherichia coli, " Virginia Journal of Science:152, 1987.
Slater et al., "Production of Poly-(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) in a Recombinant Escherichis coli Strain," Applied Env. Microbiology 58(4): 1089-1094, 1992.
Van Dyk and LaRossa, "Involvement of ack-pta operon products in .alpha.-ketobutyrate metabolism by Salmonella typhimurium," Mol. Gen. Genet. 207: 435-440, 1987.
Anderson et al., "Production of SHMT in Klebsiella aerogenes," 188th ACS National Meeting: Section MBTD, Abstract #70, 1984.
Ayers et al., eds., Microbiology of Foods, W.M. Freeman & Co., San Fran., pp. 191-192, 1980.
Ballard et al., "Formation of Polymers of .beta.-Hydroxybutyric Acid in Bacterial Cells and a Comparison of the Morphology of Growth With the Formation of Polyethylene in the Solid State," Recent Advances in Mechanistic and Synthetic Aspects of Polymerization: 293-314, D.Reidel Publishing Company, 1987.
Borel et al., "In vivo overexpression and purification of Escherichia coli tRNA.sup.ser," FEBS 324(2): 162-166, 1993.
Brown et al., "The Enzymic Interconversion of Acetate and Acetyl-coenzyme A in Escherichia coli," Journal of General Microbiology 102: 327-336, 1977.
Byrom, D., "Polymer synthesis by microorganisms: technology and economics," Trends in Biotechnology 5: 246-250, 1987.
DeBoer et al., "The tac promoter: A functional hybrid derived form the trp and lac promoters," Proc. Natl. Acad. Sci. USA 80: 21-25, 1983.
Gerdes, K., "The PARB (HOK/SOK) Locus of Plasmid R1: A General Purpose Stabilization System," Biotechnology 6: 1402-1405, 1988.
Hardesty et al., "Deletion Analysis of Sucrose Metabolic Genes form a Salmonella Plasmid Cloned in Escherichia coli K12," Plasmid 18: 142-155, 1987.
Harrison, S., "Bacteria Cell Disruption: A Key Unit Operation in the Recovery of Intracellular Products," Biotechnology Advances 9(2), 217-240, 1991.
Icho, T., "Membrane-Bound Phosphatases in Escherichia coli: Sequence of the pgpB Gene and Dual Subcellular Localization of the pgpB Product," Journal of Bacteriology 170(11): 5117-5124, 1988.
Janes et al., "Molecular Characterization of the Poly-.beta.-Hydoxybutyrate Biosynthetic Pathway of Alcaligenes eutrophus H16," Novel Biodegradable Microbial Polymers(E.A. Dawes Ed.). pp. 175-190, 1990.
Kalousek et al., "Release of Poly-.beta.-hydroxybutyrate Granules from Escherichia coli by Protein E-mediated Lysis," International Symposium on Biodegradable Polymers: p. 150, Oct. 29-31, 1991.
Kleiner et al., "Construction of Multicopy Expression Vectors for Regulated Over-production of Proteins in Klebsiella pneumoniae and Other Enteric Bacteria," Journal of General Microbiology 134: 1779-1784, 1988.
Liebergesell and Steinbuchel, "Cloning and nucleotide sequences of genes relvant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D," European Journal of Biochemistry 209(1): 135-150, 1992.
Nomura et al., "Construction of expression plasmids producing high levels of human immuno interferon in E. coli," Nucleic Acids Research, Symposium Series 12: 87-90, 1983.
Old and Primrose, eds. Principles of Gene Manipulation: An Introduction to Genetic Engineering 4th ed., Blackwell Scientific Publications, 1989, pp. 59, 153-160, 164-167.
Peoples and Sinskey, "Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding .beta.-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB," Molec. Microbio. 3: 349-357, 1989.
Peoples and Sinskey, "Poly-.beta.-hydroxybutyrate Biosynthesis in Alcaligenes eutrophus H16: Characterization of the Genes Encoding .beta.-Ketothiolase and Acetoacetyl-CoA Reductase," Journal of Biological Chemistry 264(26): 15293-15297, 1989.
Peoples and Sinskey, "Poly-.beta.-hydroxybutyrate (PHB) Biosynthesis in Alcaligenes eutrophus H16: Indentification and Characterization of the PHB Polymerase Gene (phbC)," Journal of Biological Chemistry 264: 15298-15303, 1989.
Peoples et al., "Biosynthetic Thiolase from Zoogloea ramigera," Journal of Biological Chemistry 262: 97-102, 1987.
Ploux et al., "The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera: Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli," European Journal of Biochemistry 174: 177-182, 1988.
Russell and Bennett, "Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing," Gene 20: 231-243, 1982.
Sambrook et al., eds., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, 1989, pp. 17.12-17.13, 17.17-17.21,17.27.
Schubert et al., "Cloning of the Alcaligenes eutrophus Genes for Synthesis of Poly-.beta.-Hydroxybutyric Acid (PHB) and Synthesis of PHB in Escherichia coli," Journal of Bacteriology 170: 5837-5847, 1988.
Schubert et al., "Molecular Analysis of the Alcaligenes eutrophus Poly(3-Hydroxybutyrate) Biosynthetic Operon: Identification of the N Terminus of Poly(3-Hydroxybutyrate) Synthase and Identification of the Promoter," Journal of Bacteriology 173: 168-175, 1991.
Slater et al., "Cloning and Expression in Escherichia coli of Alcaligenes eutrophus H16 Poly-.beta.-Hydroxybutyrate Biosynthetic Pathway," Journal of Bacteriology 170(10): 4431-4436, 1988.
Studier et al., "Use of T7 RNA Polymerase to Direct Expression of Cloned Genes," Methods in Enzymology 185: 60-89, 1990.
Togna et al., "Effects of Plasmid Copy Number and Runaway Plasmid Replication on Overproduction and Excretion of .beta.-Lactamas

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of production of poly-.beta.-hydroxyalkanoate copolymers does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of production of poly-.beta.-hydroxyalkanoate copolymers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of production of poly-.beta.-hydroxyalkanoate copolymers will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1370418

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.