Method of producing useful high-molecular-weight substances by c

Details Method of producing useful high-molecular-weight substances by c Method of producing useful high-molecular-weight substances by c

1992-04-17

1995-09-05

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 701, 4352402, 4351722, 4352403, 43524031, 435 702, 5303871, 530414, C12P 2108

Patent

active

054478537

ABSTRACT:
A method of producing a useful high-molecular-weight substance by culturing animal cells capable of proliferation, which comprises (A) a culturing step of culturing said animal cells in suspension to form a suspension culture mixture containing the animal cells and a useful high-molecular-weight substance produced by the animal cells; (B) a separating step of separating the animal cells from the suspension culture mixture and withdrawing the culture medium; (C) a removing step of removing low-molecular-weight substances inhibiting the growth of the animal cells from the culture medium, said inhibitor substances being the metabolites of the animal cells and different from the useful high-molecular-weight substance; (D) a recycling step of recycling part or the whole of the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culturing step (A); and (E) a recovering step of recovering the useful high-molecular-weight substance accumulated in the culture system. A culture system for culturing the animal cells is also described.

REFERENCES:
patent: 4420398 (1983-12-01), Castino
patent: 4576696 (1986-05-01), Oertli
Barnes et al. 1980 Cell 22, 649-655.
Kovar et al. 1984. Immunol. Lett. 7, 339-345.
Glacken et al. 1983 Trends Biotechnol. 1, 102-108.
Klein, J. 1982. in: Immunol. The Science of Self-Nonself Discrimination. John Wiley & Sons. New York, p. 190.
Kohler & Milstein, Nature.
Freshney, I. Culture of Animal Cells. 1983, Alan Liss Inc. New York, pp. 75 and 145.

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