Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2011-03-08
2011-03-08
Raghu, Ganapathirama (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S084000, C435S089000, C435S069700, C435S193000, C435S194000, C435S183000, C536S023200, C536S023400, C536S053000
Reexamination Certificate
active
07901912
ABSTRACT:
The present invention provides a method for enzymatically producing uridine 5′-diphospho-N-acetylgalactosamine (UDP-GalNAc) (which is an important substrate for oligosaccharide synthesis) from uridine 5′-triphosphate (UTP) and N-acetylgalactosamine 1-phosphate (GalNAc 1-P), the method including using, as an enzyme, uridine 5′-diphospho-N-acetylglucosamine pyrophosphorylase (UDP-GlcNAc pyrophosphorylase) derived from a microorganism (exclusive of a pathogenic microorganism). The GalNAc 1-P employed can be prepared from N-acetylgalactosamine and a phosphate donor in a reaction system by use of N-acetylgalactosamine kinase. According to the present invention, uridine 5′-diphospho-N-acetylgalactosamine can be efficiently produced by use of a relatively inexpensive substrate.
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Noguchi Toshitada
Okuyama Kiyoshi
K&L Gates LLP
Raghu Ganapathirama
Yamasa Corporation
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