Method of producing undifferentiated hemopoietic stem cells...

Details Method of producing undifferentiated hemopoietic stem cells... Method of producing undifferentiated hemopoietic stem cells...

2005-06-28

2005-06-28

Naff, David M. (Department: 1651)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Animal or plant cell

C424S423000, C435S177000, C435S180000, C435S347000, C435S373000, C435S395000, C435S398000, C435S289100

Reexamination Certificate

active

06911201

ABSTRACT:
A method of expanding/maintaining undifferentiated hemopoietic stem cells or progenitor cells by obtaining undifferentiated hemopoietic stem cells or progenitor cells; and either seeding the undifferentiated hemopoietic stem cells or progenitor cells into a stationary phase plug-flow bioreactor in which a three-dimensional stromal cell culture has been pre-established on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding/maintaining undifferentiated hemopoietic stem cells or progenitor cells, or culturing the undifferentiated hemopoietic stem cells or progenitor cells in conditioned medium obtained from such a reactor.

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Kadouri et al, “Dynamic Changes in Cytokine Secretion by Stromal Cells During Prolonged Maintenance Under Protein-Free Conditions”,Intl. J. Cell Cloning, 10(5):299-308, 1992.
Rosenzweig et al, “Enhanced Maintenance and Retroviral Transduction of Primitive Hematooietic Progenitor Cells Using a Novel Three-Dimensional Culture System”,Gene Therapy, 4:928-936, 1997.
Kadouri, A., “Cultivation of Anchorage-Dependent Mammalian Cells and Production of Various Metabolites”,Colloids and Surfaces B: Biointerfaces, 2:265-272, 1994.
Otsuka et al, “Expression of thec-kitLigand and Interleukin 6 Genes in Mouse Bone Marrow Stromal Cell Line”, 12(4):409-415, 1994.

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