Method of producing tissue factor pathway inhibitor

Details Method of producing tissue factor pathway inhibitor Method of producing tissue factor pathway inhibitor

1992-03-02

1993-05-18

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

530380, C12P 2102

Patent

active

052120910

ABSTRACT:
A method is disclosed for the production of tissue factor pathway inhibitor (TFPI) which comprises expression of a non-glycosylated form of TFPI in E. coli as host and obtaining a highly active TFPI by in vitro refolding of the protein in which inclusion bodies isolated from the E. coli cells are subjected to stepwise purification comprising either series (A) or series (B) as follows:
(A)

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