Method of producing substances in living silkworms

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353201, 43525233, 4352351, C12P 2100, C12N 1500, C12N 121

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active

051186160

ABSTRACT:
A method of producing useful substances which comprising propagating in cultured cells or in a host a recombinant Bombyx mori nuclear polydegrosis virus (BmNPV) DNA is disclosed. The BmNPV DNA is produced by recombination with a double-stranded DNA containing (i) a 5'-upstream BmNPV DNA fragment orginally occurring upstream from the structural gene coding for the production of polyhedral protein and also including the promoter region for the structural gene, (ii) a translational start codon and (iii) a gene coding for the production of a useful substance exogenous to the virus, with or without (iv) a 3'-downstream BmNPV DNA fragment originally occurring downstream from the structural gene coding for the production of polyhedral protein.

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