Method of producing reverse transcriptase

Details Method of producing reverse transcriptase Method of producing reverse transcriptase

1983-12-08

1988-03-08

Weimar, Elizabeth

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

4352402, 435948, C12N 900, C12N 500, C12R 191

Patent

active

047299550

ABSTRACT:
A method of producing reverse transcriptase, which comprises isolating a fraction containing retrovirus from a tissue culture fluid supernatant of retrovirus producing cells which are able to grow and propagate in vitro, treating said fraction at least once by sucrose density gradient centrifugation to thereby obtain a purified retrovirus, and extracting reverse transcriptase from said purified retrovirus.

REFERENCES:
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Moennig et al., "C-Type Particles Produced by a Permanent Cell Line from a Leukemic Pig", Virology 57, pp. 179-188 (1974).
Reid et al., "New Techniques for Culturing Differentiated Cells: Reconstituted Basement Membrane Rafts", Meth. Enzymology LVIII, pp. 263-278 (1979).
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Mizutani et al., "Endogenous RNA-Directed DNA Polymerase Activity in Virons of RNA Tumor Viruses and in a Fraction from Normal Chicken Cells", Meth. Enzym. XXIX, pp. 119-124, (1974).
Marcus et al., "Purification of Avian Myleoblastosis Virus DNA Polymerase by Affinity Chromatography on Polycytidylate Agarose", J. Virol., vol. 14(1), pp. 853-859 (1974).
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Lieber et al., `Mammalian Cells in Culture Frequently Release Type C Viruses`, Science, vol. 182, (1973), pp. 56-59.
Benveniste et al., `Multiple Divergent Copies of Endogenous C-Type Virogenes in Mammalian Cells`; Nature, vol. 252, (1974), pp. 170-173.
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