Method of producing recombinant proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252330

Reexamination Certificate

active

11113784

ABSTRACT:
A method of producing recombinant proteins, or polypeptides, in bacteria or other host cells grown on a substantially solid, i.e., solid or semi-solid, nutrient growth medium harboring DNA sequences of interest for encoding the recombinant proteins under the control of an inducer-regulated promoter or constitutive promoter. The bacteria are harvested from the solid or semi-solid nutrient medium and the desired recombinant protein is recovered by disrupting the surface of the bacteria.

REFERENCES:
patent: 5798099 (1998-08-01), Yuuki et al.
patent: 5891710 (1999-04-01), Zimmermann et al.
patent: 5945522 (1999-08-01), Cohen et al.
Wingfield et al., Current Protocols in Protein Science, Overview of the Purification of Recombinant Proteins Produced inEscherichia coli, 2002, Chapter 6.1, pp. 6.1.1-6.1.37.
Blight et al., Heterologous Protein Secretion and the VersatileEscherichia coliHaemolysin Translocator, Trends in Biotechnology, 1994, vol. 12, pp. 450-455.
Watson et al., Recombinant DNA. A Short Course, 1983, Chapter 6, Figure 6-17.
Slade et al., Recombinant Proteins—The costs of Production in D. discoideum; use of Dictyostelium discoideum as an Alternative Host to Mammal Cell Culture, Aust. Biotechnol. Conf., 1989, 8 Meet., pp. 256-258.
Bourot et al., Glycine Betaine-assisted Protein Folding in a lysA Mutant ofEscherichia coli, The Journal of Biological Chemistry, 2000, vol. 275, pp. 1050-1056.
Bosma et al., Biodegradation of 1,2,3-Trichloropropane through Directed Evolution and Heterologous Expression of a Haloalkane Dehalogenase Gene, Applied and Environmental Microbiology, 2002, vol. 68, pp. 3582-3587.
Ohno et al., “Production of a Lipopeptide Antibiotic, Surfactin, by RecombinantBcillus subtilisin Solid State Fermentation,” Biotechnology and Bioengineering, vol. 47, No. 2, Jul. 20, 1995.
Pandey et al., “Solid state fermentation for the production of industrial enzymes,” Current Science (Bangalore), (Jul. 10, 1999) vol. 77, No. 1, pp. 149-162.
Appelbaum et al., “Prokaryotic in vivo expression systems,” Chapter 6 of Protein Expression: A Practical Approach, Higgins et al., Eds., Oxford University Press, 1999.
Robinson et al., “Solid-state fermentation: A promising microbial technology for secondary metabolite production,” Applied Microbiology and Biotechnology, (Apr. 2001) vol. 55, No. 3, pp. 284-289.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of producing recombinant proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing recombinant proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing recombinant proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3860252

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.