Method of producing products with a bilayer pellet containing a

Details Method of producing products with a bilayer pellet containing a Method of producing products with a bilayer pellet containing a

1993-09-23

1995-03-14

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

435 94, 435161, 435162, 435175, 435180, 435182, C12P 100, C12N 1108, C12N 1118, C12N 1104

Patent

active

053977009

ABSTRACT:
A method of providing and sustaining a difference in pH of a first reaction of an immobilized enzyme and a second reaction in a bulk liquid surrounding the immobilized enzyme is carried out with a bilayer pellet containing coimmobilized enzymes. The pellet can contain an enzyme that produces a desired product immobilized in an inner core and urease immobilized in an outer layer. The bulk liquid contains urea and a substrate for the enzyme in the core, and has an acidic pH. The urease reacts with urea diffusing into the outer layer from the bulk liquid to produce ammonia. The ammonia consumes hydrogen ions diffusing into the inner core from the acidic bulk liquid. This provides the enzyme in the inner core with a pH higher than the acidic pH of the bulk liquid suitable for the enzyme to react with the substrate as it diffuses into the inner core. In a preferred embodiment, simultaneous isomerization of xylose to xylulose and fermentation of xylulose to ethanol is carried out with a bilayer pellet containing xylose isomerase in the core and yeast in the bulk liquid. The isomerization occurs at an optimum pH of 7.0 to 8.0 and the fermentation occurs at an optimum pH of 4.0 to 5.0.

REFERENCES:
patent: 4490468 (1984-12-01), Gong et al.
patent: 4506015 (1985-03-01), Ho et al.
Byers, et. al., Chem. Eng. Comm. vol. 112, 1992, pp. 165-187.

Affiliated with

Also associated with

Rating

Method of producing products with a bilayer pellet containing a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing products with a bilayer pellet containing a , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing products with a bilayer pellet containing a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-713233