Method of producing peptides by transforming myeloma cells with

Details Method of producing peptides by transforming myeloma cells with Method of producing peptides by transforming myeloma cells with

1987-04-14

1991-05-28

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 693, 435 694, 435 695, 435212, 435226, 4352401, C12N 1500

Patent

active

050194992

ABSTRACT:
A method of producing desired peptides by transforming mammalian cells and cultivating the transformed cells can be improved by using myeloma cells as the mammalian cells and/or using a vector having the SV40 early promoter sequence on both the 5' upstream and 3' downstream sides of the gene coding for the desired peptide.

REFERENCES:
patent: 4663281 (1987-05-01), Gillies
patent: 4713339 (1987-12-01), Levinson
patent: 4740461 (1988-04-01), Kaufman
Mulligan et al., Science, 209: 1422-1427 (1980).
Kadotani et al., Seikagaku, 56, 915 (1984).
Innis, F. et al., 1983, J. Cellular Biochem., S7A:132, Abstract of Meeting presentation.
Gillies, S. D. et al., 1983, Cell, 33: 717-728.
Macario, A. J. L. et al., "Monoclonal Antibodies Against Bacteria", vol. 1, Academic Press, p. 211 (1985).
Gray, P. W. et al., Proc. Natl. Acad. Sci. U.S.A., 80:5842-5846 (1983).

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