Method of producing peptides

Details Method of producing peptides Method of producing peptides

1994-04-22

1994-11-29

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435212, C12P 2100, C12P 2102

Patent

active

053690184

ABSTRACT:
The biocatalytic production of a peptide is carried out with a zymogen instead of the customarily used protease. Since zymogens are proteolytically inactive precursors of proteases, proteolytic side reactions are largely suppressed. Preferably, amino acid esters or peptide esters and C-terminally derivatized amino acids or peptide fragments are coupled to each other. The reaction can also take place in a frozen, aqueous medium. In particular, chymotrypsinogen, trypsinogen, pepsinogen, prorennin or procarboxypeptidase are suitable as zymogens.

REFERENCES:
patent: 3972773 (1976-08-01), Isowa et al.
Biomedica Biochemiga Acta "First International Workshop on Enzymes in Peptide Synthesis" vol. 50 1991 No. 10/11 Bielka et al. Editor pp. 1-265.
Biochemistry Kerr et al. "Catalysis By Serine Proteases and their Zymogens . . . " vol. 14 No. 23 (Nov. 18, 1975) pp. 5088-5094.

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