Method of producing particles containing nucleic acid sequences

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 911, 435 9133, 435239, 43525421, C12P 2106

Patent

active

058692878

ABSTRACT:
A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.

REFERENCES:
patent: 4879236 (1989-11-01), Smith et al.
patent: 4885248 (1989-12-01), Ahlquist
patent: 5466788 (1995-11-01), Ahlquist et al.
patent: 5500360 (1996-03-01), Ahlquist et al.
patent: 5602242 (1997-02-01), Ahlquist et al.
M. Janda and P. Ahlquist, "RNA-Dependent Replication, Transcription, and Persistence of Brome Mosaic Virus RNA Replicons in S. Cerevisiae," Cell 72:961-970, 1993.
B.H. Selling, et al., "Genomic RNA of an insect virus directs synthesis of infectious virions in plants," Proc. Natl. Acad. Sci. USA 87:434-438, 1990.
L.A. Ball, et al., "Replication of Nodamura Virus after Transfections of Viral RNA into Mammalian Cells in Culture," Journal of Virology 66(4):2326-2334, 1992.
L.A. Ball, "Replication of the genomic RNA of a positive-strand RNA animal virus from negative-sense transcripts," Proc. Natl. Acad. Sci. USA 91(26):12443-12447, 1994.
R.H. Coutts, et al., "Infection of Protoplasts from Yeast with Tobacco Mosaic Virus", Nature 240(5382):466-467, 1972.
P. Coward and A. Dasgupta, "Yeast Cells Are Incapable of Translating RNAs Containing the Poliovirus 5' Untranslated Region: Evidence for a Translational Inhibitor," Journal of Virology 66(1):286-295, 1992.
S. Das, et al., "A Small Yeast RNA Selectively Inhibits Internal Initiation of Translation Programmed by Poliovirus RNA: Specific Interaction with Cellular Proteins That Bind to the Viral 5'-Untranslated Region," Journal of Virology 68(11):7200-7211, 1994.
E.A. Emini, et al., "Production and immunological analysis of recombinant hepatitis B vaccine, " Journal of Infection 13(Suppl. A):3-9, 1986.
P.V. Hamsa and B.B. Chattoo, "Cloning and growth-regulated expression of the gene encoding the hepatitis B virus middle surface antigen in Yarrowia lipolytica," Gene 143(2):165-170, 1994.
K.J. Hofmann, et al., "Sequence Determination of Human Papillomavirus Type 6a and Assembly of Virus-like Particles in Saccharomyces cerevisiae," Virology 209(2):506-518, 1995.
K.J. Hofmann, et al., "Sequence Conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae," Journal of General Virology 77(Pt 3):465-468, 1996.
M.N. Jagadish, et al., "Expression of potyvirus coat protein in Escherichia coli and yeast and its assembly into virus-like particles," Journal of General Virology 72(pt 7):1543-1550, 1991.
Z.A. Janowicz, et al., "Simultaneous Expression of the S and L Surface Antigens of Hepatitis B, and Formation of Mixed Particles in the Methylotrophic Yeast, Hansenula polymorpha," Yeast 7(5):431-443, 1991.
J.P.M. Jore, et al., "Formation of Poliomyelitis Subrival Particles in the Yeast Saccharomyces cerevisiae,"Yeast 10(7):907-922, 1994.
R.A.Kramer, et al., "HTLV-III gag Protein is Processed in Yeast Cells by the Virus pol-protease," Science 231(4745):1580-1584, 1986.
P. Lhoas, "Mating Pairs of Saccharomyces cerevisiae infected with Double Stranded RNA Viruses from Aspergillus niger," Nature -New Biology 236(64):86-7, 1972.
R. Quadt, et al., "Formation of brone mosaic virus RNA-dependent RNA polymerase in yeast requires coexpression of viral proteins and viral RNA," Proc. Natl. Acad. Sci. USA 92(11):4892-4896, 1995.
T. Sasagawa, et al., "Synthesis and Assembly of Virus-like Particles of Human Papillomaviruses Type 6 and Type 16 in Fission Yeast Schizosaccharomyces pombe," Virology 206(1):126-135, 1995.
M.J. Schmitt and F. Neuhausen, "Killer Toxin-Secreting Double-Stranded RNA Mycoviruses in the Yeasts Hanseniaspora uvarum and Zygosaccharomyces bailii," Journal of Virology 68(3):1765-11772, 1994.
S.H. Shen, et al., "Synthesis and secretion of hepatitis B middle surface antigen by the methylotrophic yeast Hansenula polymorpha ," Gene 84(2):303-309, 1989.
K. Shiosaki, et al., "Production of hepatitis B virion-like particles in yeast ," Gene 106(2):143-149, 1991.
K. Yoshioka, et al., "Production of a unique multi-lamella structure in the nuclei of yeast expressing Drosophila copia gag precursor," FEBs Letters 302(1):5-7, 1992.
W. Zhong, "Flock House Virus, a Small Insect Ribovirus: Replication and Encapsidation of RNA2," Madison: University of Wisconsin -Madison pp. 119-138, 1993.
B.Price, et al., "Complete replication of an animal virus and maintenance of expression vectors derived from it in Saccharomyces cerevisiae," 93:9465-9470, 1996.
A. Schneemann, et al., "Use of Recombinant Baculoviruses in Synthesis of Morphologically Distinct Viruslike Particles of flock House Virus, a Nodavirus," 67(5):2756-2763, 1993.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of producing particles containing nucleic acid sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing particles containing nucleic acid sequences , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing particles containing nucleic acid sequences will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1947759

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.