Method of producing palatinose with immobilized alpha-glucosyl t

Details Method of producing palatinose with immobilized alpha-glucosyl t Method of producing palatinose with immobilized alpha-glucosyl t

1981-08-12

1983-05-31

Nafe, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435100, 435177, 435178, 435182, C12P 1918, C12P 1912, C12N 1102, C12N 1110, C12N 1104

Patent

active

043861580

ABSTRACT:
Palatinose is produced from sucrose with an immobilized bacteria containing alpha-glucosyl transferase. The bacteria is immobilized by entrapping cells of the bacteria in calcium alginate gel granules and treating the granules with polyethyleneimine and glutaraldehyde. Palatinose is efficiently produced by packing the bacteria-containing granules in a column and passing a sucrose solution through the column at high velocity.

REFERENCES:
patent: 3733205 (1973-05-01), Shorers et al.
patent: 4138292 (1979-02-01), Chibata et al.
patent: 4257884 (1981-03-01), Lim
patent: 4259445 (1981-03-01), Glass et al.
patent: 4334027 (1982-06-01), Klein et al.
patent: 4359531 (1982-11-01), Bucke et al.
Kierstan, et al., The Immobilization of Microbial Cells, Subcellular Organelles, and Enzymes in Calcium Alginate Gels, Biotech. and Bioeng., vol. XIX, 1977, (pp. 387-397).

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