Method of producing oligonucleotide arrays with features of high

Details Method of producing oligonucleotide arrays with features of high Method of producing oligonucleotide arrays with features of high

1999-10-27

2000-06-20

Woodward, Michael P.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 6, 435 912, 536 243, 536 253, C12Q 168, C12P 1934, C07H 2104, C07H 2100

Patent

active

060776749

ABSTRACT:
A method of making full-length oligonucleotide arrays provides for the purification of pre-synthesized full-length oligonucleotides from shorter length oligonucleotides and other impurities at the same time the oligonucleotides are deposited on the array. A synthesized mixture that includes desired full-length oligonucleotides and some capped shorter length or "failed" oligonucleotide sequences, is reacted with a linking agent to add a linking group on to the free-end of the full-length oligonucleotides but not the shorter-length oligonucleotides. The resulting mixture is deposited on an array without first separately purifying the mixture to remove the unwanted shorter-length oligonucleotides. After deposition, unbound material, including the shorter length oligonucleotide sequences and other impurities, is removed.

REFERENCES:
patent: 5837860 (1998-11-01), Anderson et al.
patent: 5858653 (1999-01-01), Duran et al.
Jerzy Olejnik, et al, "Photocleavable aminotag phosphoramidites for 5'-termini DNA/RNA labeling," Nucleic Acids Research, vol. 26, No. 15, 1998, pp. 3572-3576.
Alan F. Cook, et al, "Synthesis and hybridization of a series of biotinylated oligonucleotides," Nucleic Acids Research, vol. 16, No. 9, 1988, pp. 4077-4095.
Jerzy Olejnik, et al, "Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides," Nucleic Acid Research, vol. 24, No. 2, 1996, pp. 361-366.
Rainer Bischoff, et al, "Introduction of 5'-terminal functional groups into synthetic oligonucleotides for selective immobilization," Analytical Biochemistry, vol. 164, 1987, pp. 336-344.

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