Method of producing macedocin by culturing Streptococcus...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

Reexamination Certificate

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C435S071200

Reexamination Certificate

active

07449311

ABSTRACT:
The invention relates to the field of bacteriocin producing microorganisms. The invention more specifically relates to the characterization of a novel food grade bacteriocin, namely a lantibiotic, named macedocin and produced byStreptococcus macedonicusACA-DC 198. Macedocin inhibit a broad spectrum of lactic acid bacteria as well as several food spoilage and pathogenic bacteria, among othersClostridium tyrobutyricum, and is heat stable and active in a broad pH range. The invention further relates to the use of non-pathogenic microorganisms for producing macedocin in food fermentation processes, such as for preparing milk products, butter, cheese and fermented meat and vegetables, as well as in non-fermented food products such as raw meat, modified atmosphere-packed meat products, ready-to-eat meals, and canned food products, and its use for the preparation of functional starter cultures and cocultures.

REFERENCES:
patent: 5445835 (1995-08-01), Vedamuthu
patent: WO 95/06736 (1995-03-01), None
patent: WO 96/32482 (1996-10-01), None
Aktypis, et al. “Purification and Characterization of Thermophilin T, a Novel Bacteriocin Produced byStreptococcus thermophilusACA-DC 0040,”Journal of Applied Microbiology, vol. 84, pp. 568-576, 1998.
Georgalaki, et al. “Macedocin, a Food-Grade Lantibiotic Produced byStreptococcus macedonicusACA-DC-198,”Applied and Environmental Microbiology, pp. 5891-5903, Dec. 2002.
Georgalaki, et al. *“Biochemical Properties ofStreptococcus macedonicusStrains Isolated from Greek Kasseri Cheese,”Journal of Applied Microbiology, vol. 88, pp. 817-825, 2000.
Hynes, et al. “Cloning of the Gene Encoding Streptococcin A-FF22, a Novel Lantibiotic Produced bySteptococcus pyogenes, and Determination of Its Nucleotide Sequence,”Applied and Environmental Microbiology, pp. 1969-1971, Jun. 1993.
Jack, et al. “Elucidation of the Structure of SA-FF22, a Lanthionine-Containing Antibacterial Peptide Produced byStreptococcus pyogenesStrain FF22,”European Journal of Biochemistry, vol. 220, pp. 455-462, 1994.
McAuliffe, et al. “Lantibiotics: Structure, Biosynthesis and Mode of Action,”FEMS Microbiology Reviews, vol. 25, pp. 285-308, 2001.
Roberts, et al. “Shelf-Life of Pasteurized Process Cheese Spreads Made from Cheddar Cheese Manufactured with a Nisin-Producing Starter Culture,”Journal of Dairy Science, vol. 76, No. 7, pp. 1829-1836, Jul. 1993.
Taggs, et al. “Bacteriocin of a Group A Streptococcus: Partial Purification and Properties,”Antimicrobial Agents and Chemotherapy, vol. 4, No. 3, pp. 214-221, 1973.
Tsakalidou, et al. Identification ofStreptococcifrom Greek Kasseri Cheese and Description ofStreptococcus macedonicussp. nov.,International Journal of Systematic Bacteriology, vol. 48, pp. 519-527, 1998.

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