Method of producing lys-plasminogen

Details Method of producing lys-plasminogen Method of producing lys-plasminogen

1993-04-01

1994-12-06

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 681, 435183, C12P 2100, C12N 900, C12N 968

Patent

active

053710070

ABSTRACT:
To produce lys-plasminogen having a specific activity of at least 17.5 caseinolytic units/mg protein and at least 50 mymoles/g protein nitrogen as well as an electrophoretic purity of at least 90%, plasminogen from plasma, a plasminogen-containing fraction or a tissue culture is adsorbed on immobilized lysine for the purpose of purification, is eluted and is recovered from the eluate by a protein precipitating agent. A solution of the thus purified plasminogen is adjusted to a plasmin activity ranging between 0.005 and 0.2 mymoles/ml min relative to chromogenic substrate H-D-valyl-L-leucyl-L-lysine-p-nitroanilide dihydrochloride, is maintained at a temperature of from +1.degree. C. to +20.degree. C. for a period of from 6 to 60 hours in order to provoke an enzymatic-proteolytic conversion of plasminogen into lys-plasminogen, whereupon the enzymatic action is interrupted and lys-plasminogen is isolated.

REFERENCES:
Rickli et al. (1971) Biochim Biophys. Acta, 250, 447-451.
Claeys et al. (1973) Thrombosis Res, 3, 515-523.
P. Wallen and B. Wiman, Biochim. Biophys. Acta, 221, 20-30 (1970).
D. Collen et al, Thromb. Res., 7, 515-529 (1975).
Biochim. Biophys. Acta, 342, 351 (1974).
Chemical Abstracts, vol. 105, 1986, 105:22249b, "Production of Lys-plasminogen in urokinase-activated human plasma," Yamamoto et al.
Haemostatis, A. Schoppmann, et al, "Production and Quality Assurance of Lys-Plasminogen Steam Treated", vol. 18, suppl. 1, pp. 157-163, 1988.
Mammalian Biochem., Y. Makino, et al, "Conversion of Glu-Plasminogen to Lys-Plasminogen: Analysis by Immunoblotting", vol. 106, pp. 411, No. 106:117156d, 1987.
Fritz et al., Arzneim-Forsch./Drug Res. 33:479-494 (1983).
Claeys et al. Biochemica and Biophysica Acta, 342:351-359 (1974).
Badylak et al., Thrombosis Research 62:115-126 (1991).

Affiliated with

Also associated with

Rating

Method of producing lys-plasminogen does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing lys-plasminogen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing lys-plasminogen will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-213775