Method of producing long-chain alpha-hydroxyalkanoic acids

Textiles: fiber preparation

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195 79, 195 82, 195112, C12D 102, C12K 300

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040594882

ABSTRACT:
Long-chain alpha-hydroxyalkanoic acids are prepared from alpha olefins having 10 to 18 carbon atoms by first adapting a microorganism of the Candida lipolytica species to assimilate the alpha olefins in such a way that the so-adapted microorganism produces as the major metabolite of said assimilation alpha-hydroxyalkanoic acids having the same number of carbon atoms as the alpha olefins when the adapted microorganism is cultivated in an aqueous nutrient medium containing the alpha olefins. The adaptation procedure comprises first cultivating the Candida lipolytica species in an aqueous nutrient medium containing kerosene boiling range hydrocarbons and then repetitively cultivating in a series of separate successive steps in an aqueous nutrient medium containing an alpha olefin the successive microorganism cultures obtained in the preceding cultivation steps beginning with the culture obtained using the kerosene hydrocarbons as the carbon source.

REFERENCES:
patent: 3843466 (1974-10-01), Akabori et al.
Klug et al., "Fermentation of 1-Hexadecene by Candida lipolytica", Chemical Abstracts, vol. 72, No. 1, p. 191, (1970), Abs. No. 2145m.
Jwanny et al., "Utilization of Hydrocarbons by Microorganisms, Biochemical Activities of Alkanes-grown Candida lipolytica," Chemical Abstracts, vol. 81, No. 1, (1974), p. 100, Abs. No. 1199f.
Nyns et al., "Adaptive or Constitutive Nature of the Enzymes Involved in the Oxidation of n-Hexadecane to Palmitive Acid by Candida Lipolytica", Chemical Abstracts, vol. 72, No. 9, (1970), p. 123, Abs. No. 40084y.
Mishina et al., "Effects of Chain-length Alkane Substrate on Fatty Acid Composition and Biosynthetic Pathway in Some Yeasts", Agr. Biol. Chem., vol. 37, No. 4, (1973), pp. 863-870.
Waddams, Chemicals From Petroleum, The Noyes Press, Inc., Pearl River, N.Y., (1962), p. 14.

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