Method of producing interleukin-2

Details Method of producing interleukin-2 Method of producing interleukin-2

1990-06-18

1993-05-11

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 695, 435244, 530351, C12P 2102, C12N 138

Patent

active

052100294

ABSTRACT:
The present invention provides an improvement in a method for producing physiologically active proteins by cultivating Escherichia coli having an expression vector which contains a structural gene for such proteins at the downstream end of the translational starting codon. The method comprises cultivating the Escherichia coli in a medium containing (1) an iron ion source, a manganese ion source or a mixture thereof and (2) a nitrogen source derived from natural origin. The advantage of this method is an increase in the yield of physiologically active proteins substantially free of methionine (corresponding to translational starting codon ATG) at the N-terminus.

REFERENCES:
patent: 4656131 (1987-04-01), Kitano et al.
Blohm, et al., (1987) Chem. Abst. 107:174404e.

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