Method of producing insulinotropic GLP-1 (7-36) polypeptide...

Details Method of producing insulinotropic GLP-1 (7-36) polypeptide... Method of producing insulinotropic GLP-1 (7-36) polypeptide...

2004-01-20

2009-06-09

Weber, Jon P (Department: 1656)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

C435S091400, C435S091410, C435S285100, C435S070100, C530S308000, C530S344000

Reexamination Certificate

active

07544512

ABSTRACT:
The present invention discloses a method of producing polypeptides, including insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs, by ligating genes in a tandem way. Also disclosed are the recombinant polypeptides produced by such a method. Using the method of this invention, 1 to 32 copies of GLP-1 (7-36) and/or GLP-1 analog genes may be expressed in tandem and the desired polypeptide can be obtained after cleavage of a fusion protein and further processes of separation and purification thus making possible the production of recombinant polypeptides, including recombinant GLP-1 (7-36) and/or GLP-1 analogs on a large scale, at a significantly reduced production cost.

REFERENCES:
patent: 5432261 (1995-07-01), Kurono
patent: 5459049 (1995-10-01), Kurono
patent: 5512459 (1996-04-01), Wagner et al.
patent: 5545618 (1996-08-01), Buckley et al.
patent: 5994127 (1999-11-01), Selden et al.
patent: 6037143 (2000-03-01), Wagner et al.
patent: 6316224 (2001-11-01), Xia
patent: 6403361 (2002-06-01), Wagner et al.
patent: 2002/0081735 (2002-06-01), Xia
patent: 03-080096 (1991-04-01), None
patent: 06-306100 (1994-11-01), None
patent: 11-178574 (1999-07-01), None
patent: WO 95/17510 (1995-06-01), None
patent: WO 9517510 (1995-06-01), None
patent: WO 97/29180 (1997-08-01), None
patent: 98/08873 (1998-03-01), None
patent: 99/43341 (1999-09-01), None
patent: 99/43705 (1999-09-01), None
patent: WO 01/98331 (2001-12-01), None
Li et al. (2008) GLP-1 C-terminal structures affect its blood glucose lowering-function, J. Pept. Sci., vol. 14, No. 7, pp. 777-785.
Hui, H, Glucagon-Like Peptide 1 Induces Differentiation of Islet Duodenal Homeobox-1- Positive Pancreatic Ductal Cells Into Insulin-Secreting Cells, Diabetes, Apr. 2001, pp. 785-796, vol. 50.
Nielsen, J, Regulation of B-Cell Mass by Hormones and Growth Factors, Diabetes, Feb. 2001, pp. 825-829, vol. 50. Supplement 1.
Rachman, J., Normalization of Insulin Responses to Glucose by Overnight Infusion of Glucagon-Like Peptide 1 (7-36) Amide in Patients with NIDDM, Diabetes, Nov. 1996, pp. 1524-1530, vol. 45.
Tourrel, C., Persistent Improvement of Type 2 Diabetes in the Goto-Kakizaki Rat Model by Expansion of the B-Cell Mass During the Prediabetic Period With Glucagon-Like Peptide-1 or Exendin-4, Diabetes, May 2002, pp. 1443-1452, vol. 51.
Supplementary European Search Report for European Patent Application No. EP 02 75 2955, dated Jun. 15, 2005, 3 pages.

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