Method of producing infectious reovirus

Details Method of producing infectious reovirus Method of producing infectious reovirus

2006-05-23

2006-05-23

Housel, James (Department: 1648)

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

C435S325000, C435S239000, C435S239000, C435S261000, C435S006120

Reexamination Certificate

active

07049127

ABSTRACT:
A simple and efficient method of producing mammalian reovirus is developed using HEK 293 cells. The method provides for fast production of reovirus in high yield. Furthermore, this method provides for a simpler purification procedure of the produced reovirus.

REFERENCES:
patent: 4070453 (1978-01-01), Bordt et al.
patent: 4559229 (1985-12-01), Page et al.
patent: 5023252 (1991-06-01), Hsei
patent: 6136307 (2000-10-01), Lee et al.
patent: 6528305 (2003-03-01), Thompson et al.
patent: 63044532 (1988-02-01), None
patent: WO 99/08692 (1999-02-01), None
Natalie et al. Virus Research 1998, vol. 54, pp. 225-235.
Berry et al., “Production of Reovirus Type-1 and Type-3 from Vero Cells Grown on Solid and Macroporous Microcarriers,”Biotechnology and Bioengineering62, (1999), pp. 12-19.
Bos, J. L., “Ras Oncogenes in Human Cancer: A Review,”Canc. Res.,49(17), pp. 4682-4689 (1989).
Chandron and Nibert, “Protease cleavage of reovirus capsid protein mu1 and mu1C is blocked by alkyl sulfate detergents, yielding a new type of infectious subvirion particle,”J. of Virology,72(1), (1998), pp. 467-475.
Coffey, M.C., et al., “Reovirus therapy of tumors with activated Ras pathway,”Science282, (1998), pp. 1332-1334.
Hand, et al.,J. Gen. Virol.,12:121-130 (1971).
Hand, et al.,J. Mol. Biol.,82:175-183 (1974).
Drastini, Y., et al., “Comparison of eight different procedures for harvesting avian reoviruses grown in Vero cells,”J. of Virological Methods,39, (1992), pp. 269-278.
Jones, R.C., et al., “Different sensitivities of Vero cells from two sources to avian reoviruses,”Research in Veterinary Science,48, (1990), pp. 379-380.
McRae, M.A. and Joklik, W.K., “The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3,”Virology,89, (1979), pp. 578-593.
Meanger, J., et al., “Immune response to avian reovirus ain chickens and protection against experimental infection,”Aust. Vet. J.,75(6), (Jun. 1997), pp. 428-432.
Nwajei, B.N.C., et al., “Comparison of chick embryo liver and Vero cell cultures for the isolation and growth of avian reoviruses,”Avian Pathology,17, (1988), pp. 759-766.
Poggioli, et al.,J. Virol.,74:9562-9570 (2000).
Smith, R.E., et al., “Polypeptide components of virions, top component and cores of reovirus type 3,”Virology,39, (1969), pp. 791-810.
Strong, J.E. and P.W. Lee, “Thev-erBoncogene confers enhanced cellular susceptibility to reovirus infection,”J. Virol.,70, (1996), pp. 612-616.
Strong, J.E., et al., “Evidence that the Epidermal Growth Factor Receptor on Host Cells Confers Reovirus Infection Efficiency Virology,” 197(1), (1993), pp. 405-411.
Strong, J.E., et al., “The molecular basis of viral oncolysis: usurpation of the Ras signaling pathway by reovirus,”EMBO J., 17, (1998), pp. 3351-3362.
Taber et al., “The selection of virus-resistant Chinese hamster ovary cells,”Cell,8, (1976), pp. 529-533.
Tyler, et al., “Reoviruses,” inEncyclopedia of Virology,2ndedition, lines 4-14 on right column of p. 1456 (1999).
Wilcox, G.E., et al., “Adaptation and characteristics of replication of a strain of avian reovirus in Vero cells,”Avian Pathology,14, (1985), pp. 321-328.
Wyatt R.G. et al., “Probable in vitro cultivation of human reovirus like agent of infantile diarrhoea,”Lancet(1976) 1/7950:98-99.
2002 American Type Culture collection (ATCC) at http://www.atcc.org/Searchcatalogs?searchAll.cfm.
T. Orodera et al., “Recombination Between Temperature-Sensitive and Deletion Mutants of Reovirus,”Immunology,71:581-585 (1990).
D. A. Spandidos et al., “What Reassorts When Reovirus Genome Segments Reassort?”J. Virology,18:117-123 (1976).
W. K. Joklik et al., “Anti-thyroglobulin antibodies induced with recombinant reovirus infection in BALB/c mice,”J. Biol. Chem.,270:4181-4184 (1995).
Mora, M. et al., “Association of reovirus proteins with structural matrix of infected cells”, Virology 159:265-277 (1987).
Hitt, M, et al., “Construction and propagation of human adenovirus vectors”, Cell Biology vol. 1, third ed. pp. 500-512 (1998).

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