Method of producing high molecular weight sodium hyallronate by

Details Method of producing high molecular weight sodium hyallronate by Method of producing high molecular weight sodium hyallronate by

1986-01-09

1988-10-25

Weimar, Elizabeth

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435253, 435803, 435818, 435885, C12P 1904, C12N 120, C12R 146

Patent

active

047804149

ABSTRACT:
A novel mutant microorganism Streptococcus zooepidemicus HA-116 ATCC 39920, has been produced. The microorganism produces large amounts of high molecular weight hyaluronic acid. The invention provides a method of obtaining such microorganisms.
The invention also concerns a method of obtaining sodium hyaluronate which comprises growing with vigorous agitation a microorganism of the genus Streptococcus under appropriate conditions in a suitable nutrient medium containing a sugar component as a carbon source. The sugar component is present in a substantially constant concentration between 0.2 and 10 grams per liter. The medium has a substantially constant pH between about 6.0 and 7.5 and includes a substantially constant magnesium ion concentration above 0.05 grams per liter. The sodium hyaluronate excreted into the medium by the organism is purified using methods involving precipation, redissolving and reprecipating the hyaluronate. Composition of sodium hyaluronate which are characterized by an absence of pyrogenicity and skin irritation are obtained.

REFERENCES:
patent: 4517295 (1985-05-01), Bracke et al.
Buchanan et al.; `Recovery of Microorganisms from Synovial and Pleural Fluids of Animals Using Hyperosmolar Media`; Veterinary Microbiology, vol. 7 (1982); pp. 19-33.
Woolcock, J. B.; `The Capsule of Streptococcus equi`, J. of Gen. Microbial, vol. 85 (1974); pp. 372-375.
Holmstrom B. et al.; `Production of Hyaluronic Acid by a Streptococcal Strain in Batch Culture`; Appl. Microbial., vol. 15, No. 6, (1967) pp. 1409-1413.

Affiliated with

Also associated with

Rating

Method of producing high molecular weight sodium hyallronate by does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing high molecular weight sodium hyallronate by , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing high molecular weight sodium hyallronate by will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2269314