Method of producing herbicide resistant plant varieties and plan

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524051, 43524054, C12N 500

Patent

active

049006762

ABSTRACT:
A method of introducing cytoplasmically inherited traits from a donor plant into a receptor plant of the Solanaceae family has been invented. A method is provided for producing Solanum tuberosum plants which have the inherited trait. Protoplasts from the donor and receptor plants are prepared. The donor protoplasts are treated under suitable conditions to prevent extensive nuclear divisions. The treated donor protoplasts and receptor protoplasts are then contacted under suitable conditions permitting the fusion of the protoplasts. Plantlets are regenerated from the fused protoplasts, and plantlets which contain the donor cytoplasmic traits are selected and rooted. Plantlets which contain recpetor cytoplasmic traits are discarded.

REFERENCES:
Bokelmann et al., 1983, Z. Pflanzen Physiol., 109(3): 259-265.
Webb et al., 1983, Plant Science Letters, 30(1): 1-8.
Ahloowalia, B., 1982, Euphytica, 31(3): 755-759.
Miller et al., 1984, pp. 314-315, In: Handbook of Plant Cell Culture, vol. 3, (Ammirato et al., eds.), MacMillen: New York.

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